Page 76 - 《水产学报》2025年第10期

周向阳，等 水产学报, 2025, 49(10): 109605

Effects of dietary sodium acetate supplementation on growth, intestinal
health and ammonia tolerance of largemouth bass (Micropterus salmoides)

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ZHOU Xiangyang , WANG Shidong , TANG Yanjie , LI Xue , FENG Dexiang , LI Ming 1*
1. School of Marine Sciences, Ningbo University, Ningbo 315211, China;
2. School of Fisheries, Xinyang Agriculture and Forestry University, Xinyang 464000, China
Abstract: Largemouth bass (Micropterus salmoides) is an important freshwater aquaculture species; however, the intensifica-
tion of farming has increased losses attributable to ammonia stress. This study evaluated the effects of graded dietary sodium
acetate supplementation on growth performance, serum biochemical indices, digestive enzyme activities, intestinal morphology,
immune responses, inflammatory reactions, and ammonia toleranceat in M. salmoides. Fish [initial body weight (7.40 ± 0.06) g]
were randomly allocated to four treatments (three replicates, 30 fish each) and fed diets containing 0.00 (control group), 0.10%,
0.20%, and 0.40% sodium acetate, respectively, for 56 days. The results showed that supplementing the diet with sodium acet-
ate significantly enhanced the fish weight gain rate and specific growth rate, reduced the feed conversion rate; and increased
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intestinal villi length and muscle thickness, promoted the activities of intestinal lipase, pepsin, alkaline phosphatase, Na /K -
ATPase, γ-glutamyl transpeptidase and creatine kinase; and increased the contents of total protein, albumin and globulin in
serum, and reduced the contents of tumour necrosis factor, interleukin-1 and interleukin-8. After 96 h of ammonia exposure, the
cumulative mortality of M. salmoides in the sodium acetate groups was significantly lower than that of control group; the activ-
ities of serum lysozyme, total complement, total immunoglobulin, and antibody titers significantly increased; the activities of
argininesuccinate synthetase, argininosuccinate lyase, arginase, ornithine transcarboxylase and inducible nitric oxide synthase
in liver significantly increased, while the activity of neuronal nitric oxide synthase significantly decreased. The study demon-
strated that supplementing the diet with sodium acetate improved the growth performance, intestinal digestive absorption,
immune response, and ammonia tolerance of M. salmoides, indicating its potential as a feed additive for M. salmoides and a
remedy for ammonia toxicity.
Key words: Micropterus salmoides; sodium acetate; growth; ammonia
Corresponding authors: FENG Dexiang. E-mail: dxfeng@xyafu.edu.cn;
LI Ming. E-mail: liming1@nbu.edu.cn
Funding projects: National Natural Science Foundation of China (32473130, 32072948); Science and Technology Coopera-
tion Plan of Zhejiang Province (2024SNJF059)

中国水产学会主办 sponsored by China Society of Fisheries https://www.china-fishery.cn
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