Page 38 - 《水产学报》2025年第8期

陆莉莉，等 水产学报, 2025, 49(8): 089603

Analysis of DNA methylation in diploid and triploid of Crassostrea angulata

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LU Lili , XU Chengxun , KONG Lingfeng , LI Qi 1,2*
1. Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266003, China;
2. Laboratory for Marine Fisheries Science and Food Production Processes,
Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China

Abstract: DNA methylation is an important epigenetic mechanism. It can induce changes in the biological phenotype by alter-
ing gene expression and the gene expression networks involved, thereby leading to the process of adaptive evolution. At
present, epigenetic studies of polyploid genomes have been carried out in multiple aquatic animals, but epigenetic research on
the polyploid genome of Crassostrea angulata has not been reported yet. In order to explore the methylation status of genomic
DNA in different tissues of diploid and triploid C. angulata, the experiment used the fluorescence-labeled methylation sensit-
ive amplified polymorphism (F-MSAP) technology to compare and analyze genomic DNA methylation in four tissues of C.
angulata (mantle, gill, gonad, and adductor). The results showed that a total of 1 388 to 3 871 methylation sensitive sites were
detected. The total methylation levels of the mantle, gill, gonad, and adductor muscle in diploid and triploid populations were
21.05% and 21.73%, 20.44% and 18.65%, 22.04% and 22.55%, and 21.12% and 33.56%, respectively. There were extremely
significant epigenetic differences among the tissues. The full methylation levels of diploids ranged from 11.75% to 12.84%,
while the hemimethylation levels ranged from 7.60% to 9.82%, and the total methylation levels ranged from 20.44% to 22.04%.
The full methylation levels of triploids ranged from 11.26% to 30.28%, while the hemimethylation levels ranged from 3.28% to
10.47%, and the total methylation levels ranged from 18.65% to 33.56%. The total methylation level of triploids increased by
0.51%-12.44% compared with diploids. Full methylation was the predominant methylation mode, and the epigenetic differ-
ences between diploids and triploids were extremely significant. The research shows that the mantle, gill, gonad, and adductor
muscle tissues of C. angulata have different degrees of methylation levels. Methylation changes have occurred in triploid C.
angulata, and these changes may affect the way of gene expression. This study can provide reference materials for the epigen-
etic research of polyploid C. angulata.
Key words: Crassostrea angulata; diploid; triploid; DNA methylation; fluorescence-labeled methylation sensitive amplified
polymorphism (F-MSAP)

Corresponding author: LI Qi. E-mail: qili66@ouc.edu.cn
Funding projects: Agriculture Seed Improvement Project of Shandong Province (2022LZGCQY010); Key Research and
Development Program of Shandong Province (2022TZXD002)

https://www.china-fishery.cn 中国水产学会主办 sponsored by China Society of Fisheries
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