Page 151 - 《水产学报》2025年第8期

王一凡，等 水产学报, 2025, 49(8): 089613

共 2 808 bp，编码 935 个氨基酸 (aa)(图 1)。氨 基酸序列与人类 (Homo sapiens) 和小鼠等哺乳
基酸序列比对结果显示，大黄鱼 ChREBP 的氨 动物的 ChREBP 序列具有一定的同源性 (图 2)。

10 20 30 40 50 60 70 80 90
1 ATGGCGAGCAGAGGGAGAGTTTGTTCAGCCGGACAGAGCCAGCTCCAGAACTCCTCAGACTCGGACTCGGAGATGGAGCCGGTTCGGGTG
1 M A S R G R V C S A G Q S Q L Q N S S D S D S E M E P V R V
100 110 120 130 140 150 160 170 180
91 GCTTGTGGTATCGGACCGTACCCGGGTGCCTTCCCCTGCACACAGGTCATTCACAGCGGACACTTCATGGTGTCGTCACCCCACAGCGAC
31 A C G I G P Y P G A F P C T Q V I H S G H F M V S S P H S D
190 200 210 220 230 240 250 260 270
181 TCCGCGCCGCGCAGGAGGAAGAGCGGCGGATCTCTGAGATATGACTTTGACACGGTGAACAGGACCTGGTGCCAAACGTACCCATTCAGC
61 S A P R R R K S G G S L R Y D F D T V N R T W C Q T Y P F S
280 290 300 310 320 330 340 350 360
271 TCCGGGACCCTGAGCATCGACCCCACTCTGACCCGCCTGTTTGACTGCATGTCCCTGGCTTACAGTGGTAAGATAGTCTCTCCAAAGTGG
91 S G T L S I D P T L T R L F D C M S L A Y S G K I V S P K W
370 380 390 400 410 420 430 440 450
361 AAGTCTTTTAAGGGTCTGCGGTTGCTGTGGAGAGATAAGATCCGTCTGAACAATGCGATCTGGAGAGCTTGGTTCATCCAATATGTGGAG
121 K S F K G L R L L W R D K I R L N N A I W R A W F I Q Y V E
460 470 480 490 500 510 520 530 540
451 AAGAGGAAGAACACAGTTTGTGGGTTTGTCACGCCGCTGGAGGGCTCGGAGGCTGATTCACATCGGAAGCCTGAAGCGATTGTATTAGAG
151 K R K N T V C G F V T P L E G S E A D S H R K P E A I V L E
550 560 570 580 590 600 610 620 630
541 GGCAGCTACTGGAAGCGGAGGATTGAGGTGGTGATCAAGGAGTATCACAAATGGAGGATTTACTATAAGAAGAGGCTTCAGAAAAAGAAG
181 G S Y W K R R I E V V I K E Y H K W R I Y Y K K R L Q K K K
640 650 660 670 680 690 700 710 720
631 GATGATATTCTATCCATGCTACAAGAGGGTCAGATCTGCCAGGCCAAGCTGGAGAAATGGTCCAATCAGATGTACCAGGAGCCTGAGGCT
211 D D I L S M L Q E G Q I C Q A K L E K W S N Q M Y Q E P E A
730 740 750 760 770 780 790 800 810
721 GCGCCGGTCGAGGCCCACATGTTTGACCTGGACTGCCTTCTGTCTGACATCTCCGACACCCTGTTCACCATGACACAAAAGCCGAGTCCC
241 A P V E A H M F D L D C L L S D I S D T L F T M T Q K P S P
820 830 840 850 860 870 880 890 900
811 TGGGCCAGCGACCGCCACAACACGTACACGAGCAATGCTGATATAATCCAACCGGGTCTGACCCCGCTGCAGCCCAATCTGGACGATTTC
271 W A S D R H N T Y T S N A D I I Q P G L T P L Q P N L D D F
910 920 930 940 950 960 970 980 990
901 ATGGATATACCAGATATCTTTATGAACTACCGGGGCCAGCCCACTGAGCAGACTGGTTTTGCTGACTGTGGCTATTTCGAGAACCCATCC
301 M D I P D I F M N Y R G Q P T E Q T G F A D C G Y F E N P S
1000 1010 1020 1030 1040 1050 1060 1070 1080
991 AATACAGAGTTTGCTCCTGTTCCCTCCCCCGTGGTAACAGCTCCTCAGCTACTAATCGACACCCAGCTGGCTCAGCCCAGTCTGTCATCG
331 N T E F A P V P S P V V T A P Q L L I D T Q L A Q P S L S S
1090 1100 1110 1120 1130 1140 1150 1160 1170
1081 CACAGTTTGCCTCAGCCCTCCTCATCCAATACCCAGCCAGACCAGACCAGGCAGGACCAGGGATGTGCTGAATACCACACGTCTAGCATC
361 H S L P Q P S S S N T Q P D Q T R Q D Q G C A E Y H T S S I
1180 1190 1200 1210 1220 1230 1240 1250 1260
1171 ATATCAGGTGCCATACCATATGGACATCAGTCATACCCTTATCCGCCTGTTTCTATAGCATATACCACCAGCATCGAGCAGCCGGCGTCT
391 I S G A I P Y G H Q S Y P Y P P V S I A Y T T S I E Q P A S
1270 1280 1290 1300 1310 1320 1330 1340 1350
1261 TCAAGTACTCCGTTTTTGTACCCGCCTTCATGTCATAAGTCTGGTTACCACACAACAACCAGCGTGACCCCCACTGTCATCACTCACACC
421 S S T P F L Y P P S C H K S G Y H T T T S V T P T V I T H T
1360 1370 1380 1390 1400 1410 1420 1430 1440
1351 GCCTCCACCATGGGTGCCCAGAGCTCTGTACACCAGGCCCACAGCTACCCTCACACCGGCGACACATACCCCCAGACTCCCTGCCACTCT
451 A S T M G A Q S S V H Q A H S Y P H T G D T Y P Q T P C H S
1450 1460 1470 1480 1490 1500 1510 1520 1530
1441 CTCAGTTACCCGACCGCCGTGTCTGACCCGGTCCGCGCGGCTTTGCCCTCTGTCACTGTGGCCCACTGCTTCTCAGTCCCGGAGCAGGTG
481 L S Y P T A V S D P V R A A L P S V T V A H C F S V P E Q V
1540 1550 1560 1570 1580 1590 1600 1610 1620
1531 GCTGTTACAGGGGTCAGAGGGAAGCACAAGCAAAAGACAGGAGGAGTGAGGACAGCTGGTCCCCCCGTGGTTCAGCCTGGCCCCTTGTCC
511 A V T G V R G K H K Q K T G G V R T A G P P V V Q P G P L S
1630 1640 1650 1660 1670 1680 1690 1700 1710
1621 ACAGCACCCACACCCACTAGCTGCCTGGCTAAGCTGCTCTCCTCAGGCGGCCGTGAACGGAAGCCGTCGTTAGGATTTGATACTACTCTG
541 T A P T P T S C L A K L L S S G G R E R K P S L G F D T T L
1720 1730 1740 1750 1760 1770 1780 1790 1800
1711 GTGACAGCCAAAGGTCAGAGGTCAACATCTCCCAGCTCCTCGCAGTCAAGCAGCAGCACTCATGTTGGAGGATCTTCATCGCAGCTGCAG
571 V T A K G Q R S T S P S S S Q S S S S T H V G G S S S Q L Q
1810 1820 1830 1840 1850 1860 1870 1880 1890
1801 CATGGAGCCGGCTGGCCCGCAGGGATCCCAGTGGTGATACCTCTGCACAGCCAGAGTGCCACCCTGGGTCACAGTGGACCCCACGCCAGC
601 H G A G W P A G I P V V I P L H S Q S A T L G H S G P H A S
1900 1910 1920 1930 1940 1950 1960 1970 1980
1891 ACCTCCAGAGGCTCGGCGGTGTCCGCCCTGCTCAGCCACGACTCCACGACCACCGGCACTACCCTCCTCATCCCCAAGACTGAGAAACTA
631 T S R G S A V S A L L S H D S T T T G T T L L I P K T E K L
1990 2000 2010 2020 2030 2040 2050 2060 2070
1981 TCTCCCGTTCAGCTCTACAGCACAGACAGGAGCAGCCTAACAGTTAATTTTTCAGGACATCCAGGCCAAACGTCACCACCAAACCCTTTA
661 S P V Q L Y S T D R S S L T V N F S G H P G Q T S P P N P L
2080 2090 2100 2110 2120 2130 2140 2150 2160
2071 GATAAAGCCTCCAACCCAGAGTCGTCACACTCAGGCTTCTCAAGACACGGAAAGATAGAATCAAGTAAGCACACAGAGACCAGGAGGATA
691 D K A S N P E S S H S G F S R H G K I E S S K H T E T R R I
2170 2180 2190 2200 2210 2220 2230 2240 2250
2161 ACTCACATCTCTGCAGAGCAGAAGAGGCGTTTCAACATCAAACTGGGTTTTGACACTTTACATAATCTTGTGACAACACTCAGCTCTCAG
721 T H I S A E Q K R R F N I K L G F D T L H N L V T T L S S Q
2260 2270 2280 2290 2300 2310 2320 2330 2340
2251 CCAAGCATAAAGATCAGCAAAGCCACCACGCTGCAGAAGACGGCTGAGTACATCAGTAAGATGCAGCAGGAGAGAGCTCAGCTGCACGAG
751 P S I K I S K A T T L Q K T A E Y I S K M Q Q E R A Q L H E
2350 2360 2370 2380 2390 2400 2410 2420 2430
2341 GAGGCTCAGAGACTCAGAGAGGAGATCCAGCTCCTGAACTCTGCCATCAATGCGTGCCAGCAGCAGCTACCAGCCACCGGCGTGCCCATC
781 E A Q R L R E E I Q L L N S A I N A C Q Q Q L P A T G V P I
2440 2450 2460 2470 2480 2490 2500 2510 2520
2431 ACACGGCAGCGCTTTGACCACATGAGGCAGAAATTCAGAGAGTACGTCCGGGCACAGACGCTGCAGAACTGGAAGTTCTGGATCTTCAGT
811 T R Q R F D H M R Q K F R E Y V R A Q T L Q N W K F W I F S
2530 2540 2550 2560 2570 2580 2590 2600 2610
2521 ATAATCATCGAGCCGTTGTTTGAGTCCTATAATGGGATGGTGTCCACTGCCAGCGTGGAGGACCTGTGTCGATCCACTCTGTCCTGGCTG
841 I I I E P L F E S Y N G M V S T A S V E D L C R S T L S W L
2620 2630 2640 2650 2660 2670 2680 2690 2700
2611 GATCAACACTGTTCCCTGCCTGCCCTCAGACCCATGGTTTTGAGTTCACTCCGTCTCCTGAGTACAACCACGGCCATCCTGTCAGACCCC
871 D Q H C S L P A L R P M V L S S L R L L S T T T A I L S D P
2710 2720 2730 2740 2750 2760 2770 2780 2790
2701 AGCCTACTGCCGGAGCAGGCCACCCTCGCTGTCACCCAGGGCGACGTTGCTGCTACCCTGGACCACTCCTTACAGCCCGCCACCCGGGAC
901 S L L P E Q A T L A V T Q G D V A A T L D H S L Q P A T R D
2800
2791 AACATACACCCCATGTGA
931 N I H P M *
图 1 大黄鱼 ChREBP 的 CDS 序列和氨基酸序列
Fig. 1 CDS and deduced amino acid sequence of L. crocea ChREBP
https://www.china-fishery.cn 中国水产学会主办 sponsored by China Society of Fisheries
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