Page 64 - 《水产学报》2025年第6期

梁肖，等 水产学报, 2025, 49(6): 069605

表 2 LV-sox9-shRNA 侵染 ZZ 胚胎后性腺的雌雄数量及性逆转率
Tab. 2 The number of male and female gonads and the rate of sex reversal after LV-sox9-shRNA infection with ZZ embryos
时期 组别 胚胎数/个 睾丸数/个 卵巢数/个 卵睾丸数/个 性逆转率
stages groups no. of embryos no. of testes no.of ovaries no. of ovotestes sex reversal rate
第21期 对照组ZZ 34 34 0 0 —
stage 21
ZZ+LV-sox9-shRNA 15 3 10 2 (10+2)/15
对照组ZW 35 0 35 0 —
第27期 对照组ZZ 42 42 0 0 —
stage 27
ZZ+LV-sox9-shRNA 33 4 29 0 (29+0)/33
对照组ZW 39 0 39 0 —
注：雄性向雌性逆转率=(ZZ卵巢数+ZZ卵睾丸数)/ZZ胚胎数；通过性腺的表型观察以及雌雄蛋白的免疫荧光染色判断；“—” 表示对照组不存
在性逆转；下同。
Notes: Male-to-female sex reversal rate=(no. of ZZ ovaries and ZZ ovotestes)/total no. of ZZ embryos; the reversed gonads were assessed by phenotypic
observation of gonads and immunofluorescence staining of male and female proteins; “—” indicates that there is no sex reversal in the control group; the
same below.

ZW ZW+sox9-OE ZZ

50 μm 50 μm 50 μm 50 μm
1 2 3 4
gc gc Cor
Cor gc
Cor
gc gc sc
Med sc Med sc
Med Med
gc
50 μm 5 50 μm 6 50 μm 7 50 μm 8

图版 Ⅳ sox9 过表达后第 27 期 ZW 胚胎性腺的表型变化
Plate Ⅳ Phenotypic changes of ZW embryonic gonads of stage 27 after sox9 overexpression

1.5 n.s. **
dmrt1 基因相对表达量 relative expression level of dmrt1 1.0 *** amh 基因相对表达量 relative expression level of amh 1.5 n.s. ** foxl2 基因相对表达量 relative expression level of foxl2 1.5
1.0
1.0

0.5
0.5
0.5

0
1 n.s. 2 3 4 0 1 2 3 4 0 1 2 3 4
实验组别 实验组别 实验组别
experimental group experimental group experimental group
(a) (b) (c)
图 3 sox9 过表达后第 27 期 ZW 胚胎性腺中雌雄特异性基因的表达变化
Fig. 3 Expression changes of male- and female-specific genes in ZW embryonic gonads of
stage 27 after sox9 overexpression
1. ZW nor, 2. ZW control, 3. ZW+sox9-OE, 4. ZZ control.

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