Page 143 - 《水产学报》2023年第1期

李晓晖，等 10 C A 20 30 水产学报, 2023, 47(1): 019610

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图 1 miR-17a 的二级结构
加粗部分为 miR-7a 的成熟序列，红色部分为 HIF 结合位点
Fig. 1 Secondary structure of miR-17a
The bold part is the mature sequence of miR-7a, and the red part is the HIF binding site
表 3 不同物种 miR-17 的登录号及成熟序列
Tab. 3 Accession number and mature sequence of miR-17 in different species
物种 拉丁名 登录号 成熟序列
species Latin names accession miRNA ID mature sequence
鲤 Cyprinus carpio MI0023342 ccr-mir-17 CAAAGUGCUUACAGUGCAGGUAG
斑马鱼 Danio rerio MI0001897 dre-mir-17a-1 CAAAGUGCUUACAGUGCAGGUA
斑点叉尾鮰 Ictalurus punctatus MI0024524 ipu-mir-17a CAAAGUGCUUACAGUGCAGGUA
红鳍东方鲀 Fugu rubripes MI0003231 fru-mir-17-1 CAAAGUGCUUACAGUGCAGGUA
青斑河鲀 Tetraodon nigroviridis MI0003232 tni-mir-17-1 CAAAGUGCUUACAGUGCAGGUA
大西洋鲑 Salmo sala MI0026535 ssa-mir-17-1 CAAAGUGCUUACAGUGCAGGUAG
大西洋鳕 Gadus morhua MI0036194 gmo-mir-17-1 CAAAGUGCUUACAGUGCAGGUA
青鳉 Oryzias latipes MI0019542 ola-mir-17 CAAAGUGCUUACAGUGCAGGUA
热带爪蟾 Xenopus tropicalis MI0004803 xtr-mir-17 CAAAGUGCUUACAGUGCAGGUAGU
斑胸草雀 Taeniopygia guttata MI0013784 tgu-mir-17a CAAAGUGCUUACAGUGCAGGUAG
人类 Homo sapiens MI0000071 hsa-mir-17 CAAAGUGCUUACAGUGCAGGUAG
小鼠 Mus musculus MI0000687 mmu-mir-17 CAAAGUGCUUACAGUGCAGGUAG
倭狐猴 Microcebus murinus MI0039918 mmr-mir-17 CAAAGUGCUUACAGUGCAGGUAG
白颊长臂猿 Nomascus leucogenys MI0040207 nle-mir-17 CAAAGUGCUUACAGUGCAGGUAG
穴兔 Oryctolagus cuniculus MI0039225 ocu-mir-17 CAAAGUGCUUACAGUGCAGGUAG
山羊 Capra hircus MI0030648 chi-mir-17 CAAAGUGCUUACAGUGCAGGUAGU
九带犰狳 Dasypus novemcinctus MI0038909 dno-mir-17 CAAAGUGCUUACAGUGCAGGUAG
眼镜王蛇 Ophiophagus hannah MI0031372 oha-mir-17 CAAAGUGCUUACAGUGCAGGUAGU
树鼩 Tupaia chinensis MI0031174 tch-mir-17 CAAAGUGCUUACAGUGCAGGUAG
蟒蛇 Python bivittatus MI0030161 pbv-mir-17 CAAAGUGCUUACAGUGCAGGUA
原鸽 Columba livia MI0029912 cli-mir-17 UCCAGUGAUUCACACCAGCUGU
原鸡 Gallus gallus MI0001184 gga-mir-17 UCGCCUUCCCUUCCCGCCCUUC

建了野生型 (WT-pmirGLO-HIF-1α 3′UTR) 和突变 证 实 了 miR-17a-5p 可 以 靶 向 结 合 HIF-1α 基 因
型 (MUT-pmirGLO-HIF-1α 3′UTR) 双荧光素酶报 3′UTR 区域序列。
告基因载体。细胞转染后发现，仅 WT-pmirGLO- 通过荧光定量 PCR 分析 miR-17a-5p 和 HIF-
HIF-1α 3′UTR 与 mimics miR-17a-5p 共转染时相对 1α 在低氧胁迫下不同组织中的相对表达。结果显
荧光素酶活性显著降低 (P<0.05)，其余各转染组 示，在不同程度的低氧胁迫下，miR-17a-5p 的表
间的相对荧光素酶活性无显著变化 (P>0.05) (图 5-b)， 达均低于对照组 (图 6)，HIF-1α 的表达均高于对

中国水产学会主办 sponsored by China Society of Fisheries https://www.china-fishery.cn
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