Page 100 - 《中国药科大学学报》2025年第5期

632 学报 Journal of China Pharmaceutical University 2025, 56(5): 624 − 633 第 56 卷
A B C

3 5
WKY SHR SHR+Z **
JAK2 131 kD 2 * 4

p-JAK2 131 kD Ratio of p-AKT1/AKT1 # Ratio of p-PI3K/PI3K 3 2 #

STAT3 88 kD 1 1

p-STAT3 88 kD 0 0
WKY SHR WKY SHR
PI3K 85 kD SHR＋ZTKCW SHR＋ZTKCW

p-PI3K 84 kD D E
3 3 *
Ratio of p-STAT3/STAT3 1 Ratiof o p-JAK2/JAK2 1
AKT1 56 kD **
p-AKT1 56 kD 2 # 2 #

0
WKY SHR 0 WKY SHR
SHR＋ZTKCW SHR＋ZTKCW

Figure 5 Relative protein expression of AKT serine/threonine kinase 1 (AKT1), p-AKT1, phosphatidylinositol 3-kinase (PI3K), p-PI3K,
signal transducer and activator of transcription 3 (STAT3), p-STAT3, Janus kinase 2 (JAK2) and p-JAK2 in heart tissues of rats in WKY
group (control), SHR group, and SHR + Z (SHR+ZTKCW-H) group (1.64 g/kg)
A: Protein expression of AKT1, p-AKT1, PI3K, p-PI3K, STAT3, p-STAT3, JAK2 and p-JAK2 were detected by Western blot; B-E:
Quantitative graph of p-AKT1/AKT1, p-PI3K/PI3K, p-STAT3/STAT3 and p-JAK2/JAK2 ( x ± s, n=3)
#
*P<0.05, **P<0.01 vs WKY group, P<0.05 vs SHR group
A WKY SHR SHR+ZTKCW B WKY SHR SHR+ZTKCW

AKT1 p-AKT1
20 μm 20 μm

PI3K p-PI3K
20 μm 20 μm

STAT3 p-STAT3
20 μm 20 μm

JAK2 p-JAK2
20 μm 20 μm

Figure 6 Immunofluorescence staining of thoracic aorta of rats in WKY group (control), SHR group, and SHR+ZTKCW-H group
(1.64 g/kg)
A: Representative images of AKT1, PI3K, STAT3, and JAK2 immunofluorescence staining of thoracic aorta of rats; B: Representative
images of p-AKT1, p-PI3K, p-STAT3, and p-JAK2 immunofluorescence staining of thoracic aorta of rats

产生大量的过氧化物，加剧血管内皮细胞（vascular eNOS 是控制内皮 NO 生成的重要分子，JAK2 通过
endothelial cell， VEC） 的 氧 化 应 激 和 功 能 障 碍 。 激 活 转 录 因 子 Sp-1 促 进 eNOS 表 达 ， 从 而 维 持