Page 17 - 《渔业研究》2026年第3期

310 渔 业 研 究 第 48 卷

Genes expression and metabolites levels of F generation of selected and
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non-selected lines of landlocked Oncorhynchus masou eggs based on
transcriptomics and metabolomics analysis

1*
2
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JIN Xiaoyan ，HAN Mingming ，PENG Sibo ，WANG Guiqin ，
1
1
1
1
CHEN Xiumei ，NIU Xiaotian ，KONG Yidi ，LI Min 1
(1. Key Laboratory for Animal Production, Product Quality and Safety of Ministry of Education, International Joint Laboratory of
Modern Agricultural Technology of the Ministry of Education, College of Animal Science and Technology,
Jilin Agriculture University, Changchun 130118, China;
2. Jilin Provincial Academy of Fishery Sciences, Changchun 130033, China)
Abstract: [Objectives] The effects of growth trait selection on the genes expression and metabolites levels
in the eggs of landlocked Oncorhynchus masou were investigated by transcriptomics and metabolomics.
[Methods] Eggs were collected from 15 non-selected and 15 fifth-generation (F ) selected landlocked O. masou.
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Differences in gene expression and metabolites levels between the eggs of the two species were analyzed by
combining transcriptomics and metabolomics. [Results] The results of the transcriptome analysis revealed a
total of 10 880 differentially expressed genes (DEGs), primarily enriched in ribosomes, oxidative phosphoryla-
tion, lysosomes, and FoxO signaling pathways. Among these, the selected line of landlocked O. masou exhib-
ited upregulated expression of gonadotropin (Gn), gonadotropin-releasing hormone (GnRH), and follicle-stimu-
lating hormone (FSH) genes associated with oogenesis promotion, while downregulated expression of gonado-
tropin-inhibiting hormone (GnIH) genes related to oogenesis inhibition. The results of the metabolomics analys-
is revealed 583 differentially expressed metabolites (DEMs). Among these, the levels of vitellin (Vn), alkaline
phosphatase (AKP), malate dehydrogenase (MDH), α-tocopherol, docosahexaenoic acid (DHA), and eicos-
apentaenoic acid (EPA) were higher in the selected group compared to the non-selected group. Conversely, the
levels of vitamin B , spermine, and adenosine were lower in the selected group compared to the non-selected
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group. The combined analysis revealed that phospholipid, lysine, glucose and fatty acid metabolic signaling
pathways participate in ovarian development by enhancing lipid and fatty acid matabolism. [Conclusions] Con-
tinuous selection had significant effects on gene expression and metabolite levels of landlocked O. masou eggs,
and phospholipid, lysine, glucose and fatty acid metabolic signaling pathways played important roles in the de-
velopment of ovarian and eggs.
Key words: Oncorhynchus masou; landlocked population; fish egg; selected and non-selected lines;
transcriptome-metabolome joint analysis