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TaggedEnd198                                                                                     J. Liao et al.
         adjusting for sociodemographic and lifestyle factors and  conditions. Among females, those engaged in insufficient
         chronic conditions (Table 2). Specifically, females and males  LTPA also had lower total fat (0.63%, 95%CI: 1.10% to
         who spent more than 8 h/day sitting had 1.97% (95%CI:  0.15%) and trunk fat (0.67%, 95%CI: 1.31% to 0.04%)
         1.39%2.55%) and 1.84% (95%CI: 1.06%2.62%) higher    than those who were inactive, but this association was attenu-
         total fat, respectively, compared with those who spent less  ated to null after additionally adjusting for lifestyle behaviors
         than 4 h/day sitting. Meanwhile, females and males who spent  and chronic conditions (total fat: 0.47%, 95%CI: 0.97% to
         more than 8 h/day sitting had 1.91% (95%CI: 1.11%2.70%)  0.03%; trunk fat: 0.55%, 95%CI: 1.22% to 0.13%). Similar
         and 2.03% (95%CI: 1.16%2.90%) higher trunk fat, respec-  findings were observed in males who engaged in insufficient
         tively. A significant doseresponse relationship was exhibited  LTPA.TaggedEnd
         between daily sitting time and body fat percentage, such that
         each 1 h/day increase in daily sitting time was associated with
         0.23%   (95%CI:  0.17%0.29%)  and  0.20%   (95%CI:   TaggedH23.3. Daily sitting time and LTPA with body fatTaggedEnd
         0.13%0.27%) higher total body fat among females and
                                                                  TaggedPIn the analysis stratified by LTPA, prolonged daily sitting
         males, respectively, while these increases were 0.23%
                                                               time was associated with higher total and trunk fat among
         (95%CI: 0.14%0.31%) and 0.22% (95%CI: 0.14%0.30%)
                                                               adults who were physically active and inactive/insufficiently
         for trunk fat.TaggedEnd
                                                               active (Table 3). Compared to sitting less than 4 h/day, sitting
                                                               more than 8 h/day was associated with 1.75% (95%CI:
         TaggedH23.2. LTPA and body fatTaggedEnd
                                                               0.75%2.75%) and 1.47% (95%CI: 0.55%2.38%) higher
            TaggedPBeing physically active was associated with lower total and  total fat and 1.65% (95%CI: 0.44%2.87%) and 1.71%
         trunk fat percentages (Table 2). Adults engaging in sufficient  (95%CI: 0.73%2.70%) higher trunk fat among physically
         LTPA tended to have lower total (female: 2.15%, 95%CI:  active females and males, respectively. For those who were
         2.69% to 1.61%; male: 1.98%, 95%CI: 2.52% to      inactive/insufficiently active, sitting more than 8 h/day was
         1.45%) and trunk (female: 2.26%, 95%CI: 2.96% to   associated with 1.91% (95%CI: 1.25%2.58%) and 2.13%
         1.57%; male: 2.12%, 95%CI: 2.71% to 1.52%) fat    (95%CI: 1.04% 3.23%) higher total fat and 1.86% (95%CI:
         compared with those who were inactive after adjusting for  0.92%2.81%) and 2.24% (95%CI: 1.04% to 3.43%) higher
         sociodemographic factors, lifestyle behaviors, and health  trunk fat among females and males, respectively.TaggedEnd


         TaggedEndTable 2
         Association of daily sitting time and LTPA with total and trunk fat percentages among U.S. adults 20 years, NHANES 20112018.
                                                                b-coefficient (95%CI)
                                          Total fat percentage                        Trunk fat percentage
                          MV Model 1 a   MV Model 2 a,b  MV Model 3 a,b,c  MV Model 1 a  MV Model 2 a,b  MV Model 3 a,b,c
         Male
           Daily sitting time (h/day)
            <4            0 (reference)  0 (reference)  0 (reference)  0 (reference)  0 (reference)  0 (reference)
            4<6          0.54 (0.06 to 1.14)  0.68 (0.08 to 1.29)  0.53 (0.11 to 1.18)  0.70 (0.03 to 1.37)  0.87 (0.19 to 1.55)  0.66 (0.05 to 1.38)
            68           1.49 (0.88 to 2.11)  1.42 (0.78 to 2.07)  1.30 (0.64 to 1.97)  1.65 (0.97 to 2.33)  1.57 (0.86 to 2.28)  1.44 (0.70 to 2.19)
            >8            1.90 (1.17 to 2.62)  1.87 (1.11 to 2.63)  1.84 (1.06 to 2.62)  2.05 (1.22 to 2.89)  2.07 (1.20 to 2.93)  2.03 (1.16 to 2.90)
           Per 1 h/day increase  0.21 (0.14 to 0.28)  0.20 (0.13 to 0.27)  0.20 (0.13 to 0.27)  0.22 (0.14 to 0.30)  0.21 (0.13 to 0.30)  0.22 (0.14 to 0.30)
           LTPA
            Inactive      0 (reference)  0 (reference)  0 (reference)  0 (reference)  0 (reference)  0 (reference)
            Insufficiently active  0.65 (1.43 to 0.13)  0.56 (1.31 to 0.19)  0.51 (1.24 to 0.21)  0.73 (1.63 to 0.16)  0.64 (1.48 to 0.20)  0.66 (1.47 to 0.15)
            Physically active  2.41 (2.96 to 1.86) 2.13 (2.68 to 1.59) 1.98 (2.52 to1.45)  2.55 (3.15 to 1.95) 2.26 (2.87 to 1.64) 2.12 (2.71 to 1.52)
         Female
           Daily sitting time (h/day)
            <4            0 (reference)  0 (reference)  0 (reference)  0 (reference)  0 (reference)  0 (reference)
            4<6          0.56 (0.01 to 1.12)  0.62 (0.08 to 1.16)  0.53 (0.04 to 1.10)  0.63 (0.06 to 1.33)  0.70 (0.03 to 1.37)  0.65 (0.07 to 1.38)
            68           1.45 (0.89 to 2.00)  1.39 (0.85 to 1.94)  1.24 (0.73 to 1.75)  1.62 (0.90 to 2.34)  1.55 (0.87 to 2.24)  1.32 (0.67 to 1.98)
            >8            2.22 (1.64 to 2.80)  2.17 (1.61 to 2.73)  1.97 (1.39 to 2.55)  2.29 (1.47 to 3.12)  2.19 (1.40 to 2.98)  1.91 (1.11 to 2.70)
           Per 1 h/day increase  0.26 (0.19 to 0.32)  0.25 (0.18 to 0.31)  0.23 (0.17 to 0.29)  0.27 (0.18 to 0.36)  0.26 (0.17 to 0.34)  0.23 (0.14 to 0.31)
           LTPA
            Inactive      0 (reference)  0 (reference)  0 (reference)  0 (reference)  0 (reference)  0 (reference)
            Insufficiently active  0.63 (1.10 to 0.15) 0.55 (1.05 to 0.05) 0.47 (0.97 to 0.03)  0.67 (1.31 to 0.04) 0.57 (1.22 to 0.08)  0.55 (1.22 to 0.13)
            Physically active  2.77 (3.27 to 2.26) 2.34 (2.87 to 1.82) 2.15 (2.69 to 1.61) 3.05 (3.73 to 2.37) 2.50 (3.19 to 1.80) 2.26 (2.96 to 1.57)
         a
           Multivariable model additionally adjusted for age (year), sex (male or female), race (non-Hispanic White, non-Hispanic Black, Hispanic, or other), educational
         attainment (less than high school, high school graduate, or above high school), and family poverty ratio (<1.3, 1.3 to <3.5, or 3.5).
         b
           Additionally adjusted for smoking status (never, former, or current), alcohol use (never, former, or current), total energy intake, and Healthy Eating Index-2015.
         c
           Additionally adjusted for hypertension (yes or no), hypercholesterolemia (yes or no), history of diabetes (yes or no), history of CVD (yes or no), history of cancer
         (yes or no), and depression (yes or no).
         Abbreviations: 95%CI = 95% confidence interval; CVD = cardiovascular disease; LTPA = leisure-time physical activity; MV = multivariable; NHANES = National
         Health and Nutrition Examination Survey.
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