Page 109 - 《水产学报》2026年第3期

3 期 王笑然，等：慢性热应激诱导大口黑鲈肝脏线粒体功能障碍及糖脂异常沉积 50 卷

EVM0002015 E
V
M
0
0
0
4
1
5
生物学过程 biological process EVM0000255 0
EVM0001227
EVM0000462
EVM0000062
1 EVM0001404
2
3 EVM0000573
4
5 q-value EVM0000655
6 0.001 00
0.000 75
7 0.000 50 EVM0000459
8 0.000 25 EVM0001484
9
EVM0000135
10 gene_number EVM0000519 4 6
11
12 40 EVM0000154 0 0 7
80
13 120 EVM0000745 0 logFC
14 diff 0 M
15 up V −2 3
16 up&down GO Terms E
17
18 免疫反应 免疫应答的调节
19 immune response regulation of immune response
20 细胞黏附的正向调控 免疫系统过程调控
1.5 2.0 2.5 3.0 positive regulation of cell adhesion regulation of immune system process
细胞黏附调控
细胞激活调控
富集因子 regulation of cell adhesion regulation of cell activation
免疫系统过程
整合素介导的信号通路
rich factor immune system process integrin-mediated signaling pathway
(a) 细胞运动的正向调控 细胞表面受体信号通路
cell surface receptor signaling pathway
positive regulation of cell motility
(b)
生物学过程 biological process
1 fold change
2 5.0
3 2.5
4 q-value 0
5 −2.5
6 0.007 5 −5.0
7 0.005 0 size
0.002 5
8 40
50
9
10 gene_number 60
11 20 70
40
80
12 60
13 80
14 diff
15 down
16 up
17 up&down
18 类别 category
19 碳代谢
20 C 型凝集素受体信号通路 carbon metabolism
C-type lectin receptor signaling pathway
2 4 6 细胞因子-细胞因子受体相互作用 糖酵解/糖异生
富集因子 cytokine-cytokine receptor interaction glycolysis/gluconeogenesis
肌动蛋白细胞骨架调控
rich factor regulation of actin cytoskeleton
(c) (d)

图 6 CHS 下大口黑鲈肝脏转录组结果 GO 及 KEGG 通路富集分析
(a) DEGs GO 分类气泡图，图中 1~20 分别为：1.免疫应答；2.免疫应答的调节；3.细胞黏附的正向调控；4.细胞黏附调控；5.免疫系统过程
调控；6.细胞激活调控；7.免疫系统过程；8.细胞运动的正向调控；9.整合素介导的信号传导；10.细胞表面受体信号通路；11.细胞成分迁
移的正向调控；12.细胞运动的调控；13.细胞迁移的调控；14.细胞成分迁移的调控；15.细胞迁移的正向调控；16.免疫系统过程的正向调
控；17.小分子分解过程；18.白细胞分化；19.运动调节；20.细胞激活的正向调控；(b) GO 前 10 聚类通路；(c) DEGs KEGG 通路富集分析
气泡图，图中 1~20 分别为：1. 细胞因子-细胞因子受体相互作用；2.碳代谢；3.糖酵解/糖异生；4.肌动蛋白细胞骨架调控；5. C 型凝集素
受体信号通路；6.色氨酸代谢；7.爱泼斯坦-巴尔病毒感染；8.丙酮酸代谢；9.细胞色素 P450 对外源性物质的代谢；10.叶酸介导的单碳库；
11.甘氨酸、丝氨酸和苏氨酸的代谢过程；12.脂肪酸分解；13.黏着斑；14. FcγR 介导的吞噬作用；15.缬氨酸、亮氨酸和异亮氨酸的分解过
程；16.氨基酸的生物合成；17.柠檬酸循环 (三羧酸循环)；18.果糖和甘露糖代谢；19.抗坏血酸和醛糖酸代谢；20.戊糖与葡萄糖醛酸相互转
化途径；(d) KEGG 前 5 富集通路。
Fig. 6 GO and KEGG pathway enrichment analysis of liver transcriptome in M. salmoides under CHS
(a) GO classification bubble charts of DEGs, items 1 to 20 in the figure are as follows: 1. immune response; 2. regulation of immune response; 3. posit-
ive regulation of cell adhesion; 4. regulation of cell adhesion; 5. regulation of immune system process; 6. regulation of cell activation; 7. immune system
process; 8. positive regulation of cell motility; 9. integrin-mediated signaling pathway; 10. cell surface receptor signaling pathway; 11. positive regula-
tion of cellular component movement; 12. regulation of cell motility; 13. regulation of cell migration; 14. regulation of cell migration; 15. positive regula-
tion of cell migration; 16. positive regulation of immune system process; 17. small molecule catabolic process; 18. leukocyte differentiation; 19. regula-
tion of locomotion; 20. positive regulation of cell activation; (b) the top 10 clustered pathways; (c) KEGG pathway enrichment analysis bubble charts of
DEGs, items 1 to 20 in the figure are as follows: 1. cytokine-cytokine receptor interaction; 2. carbon metabolism; 3. glycolysis/gluconeogenesis; 4. regu-
lation of actin cytoskeleton; 5. c-type lectin receptor signaling pathway; 6. tryptophan metabolism; 7. Epstein-Barr virus infection; 8. pyruvate metabol-
ism; 9. metabolism of xenobiotics by cytochrome P450; 10. folate-mediated one-carbon pool; 11. glycine, serine and threonine metabolism; 12. fatty acid
degradation; 13. focal adhesion; 14. FcγR-mediated phagocytosis; 15. valine, leucine and isoleucine degradation; 16. biosynthesis of amino acids; 17. cit-
rate cycle (TCA cycle); 18. fructose and mannose metabolism; 19. ascorbate and aldarate metabolism; 20. pentose and glucuronate interconversions; (d)
the top 5 enriched KEGG pathways.
中国水产学会主办 sponsored by China Society of Fisheries https://www.china-fishery.cn
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