Page 204 - 《水产学报》2026年第2期

2 期 水 产 学 报 50 卷

A comparative study on the molecular characteristics, prokaryotic
expression and antiviral function of ifi27l2a between grass carp
(Ctenopharyngodon idella) and barbel chub (Squaliobarbus curriculus)

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XIONG Shuting , CHEN Youcheng , CHEN Haitai ,
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HUANG Chao , CAO Bin , XIAO Tiaoyi 1*
(1. College of Fisheries, Hunan Agricultural University, Changsha 410128, China;
2. Xiangyin Institute of Aquatic Sciences, Xiangyin 414000, China;
3. Xiangtan County Bureau of Agriculture and Rural Development, Xiangtan 411228, China)

Abstract: To investigate the functional properties of interferon, alpha-inducible protein 27 like 2 a (ifi27l2a) and the differ-
ences in the mechanisms of anti-grass carp reovirus (GCRV) between C. idella and S. curriculus, this study applied cloning,
multiple sequence alignment, prokaryotic expression, qPCR and other methods to compare the sequence characteristics, expres-
sion characteristics and antiviral function of ifi27l2a between C. idella and S. curriculus. The results showed that the open read-
ing frames of ifi27l2a in C. idella and S. curriculus are both 288 bp, encoding 95 amino acids, with high sequence homology,
but there are differences in the β-sheets. Ifi27l2a is widely distributed in various tissues of C. idella and S. curriculus. After
GCRV infection, it is significantly induced in the liver, spleen, and kidney of C. idella, but only in the heart of S. curriculus,
and the induction expression in C. idella is much higher than that in S. curriculus. Meanwhile, in the kidney cell lines of C.
idella and S. curriculus, the induced expression level of ifi27l2a in CIK at 12 h post GCRV infection was significantly higher
than that in SCK, which was similar to the results of the individual, suggesting that the differences in the expression pattern and
the induced expression of ifi27l2a might be an important factor in the differences in the resistance ability between C. idella and
S. curriculus. To compare the antiviral function of the IFI27L2A protein in C. idella and S. curriculus, the recombinant pro-
teins rScIFI27L2A and rCiIFI27L2A were successfully prepared in this experiment. The results of virus infection and protein
incubation experiments confirmed that both can effectively inhibit GCRV replication. It is worth mentioning that the recombin-
ant protein rScIFI27L2A significantly inhibits the IFN response induced by GCRV in SCKs, while the rCiIFI27L2A signific-
antly enhances the IFN response in CIKs, indicating that there are significant differences in the antiviral regulatory mechan-
isms of IFI27L2A in C. idella and S. curriculus. Our research results provide clues for the molecular mechanism of resistance
difference to GCRV in C. idella and S. curriculus, and provide theoretical references for the development of antiviral drugs for
fish and in-depth research on fish antiviral mechanisms.
Key words: Ctenopharyngodon idella; Squaliobarbus curriculus; IFI27L2A; prokaryotic expression; differences in regulatory
mechanisms
Corresponding author: XIAO Tiaoyi. E-mail: tiaoyixiao@hunau.edu.cn
Funding projects: Biological Breeding-National Science and Technology Major Project (2023ZD04065); Hunan Provincial
Natural Science Foundation (2024JJ5200); Scientific Research Fund of Hunan Provincial Education Department (24B0192)

https://www.china-fishery.cn 中国水产学会主办 sponsored by China Society of Fisheries
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