Page 194 - 《水产学报》2026年第01期

1 期 水 产 学 报 50 卷

Effects of dietary resveratrol supplementation on growth performance,
antioxidant response, and ammonia detoxification in
largemouth bass (Micropterus salmoides)

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2*
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YUE Yingqi , WANG Shidong , TANG Yanjie , FENG Dexiang , LI Ming 1*
(1. School of Marine Sciences, Ningbo University, Ningbo 315211, China;
2. School of Fisheries, Xinyang Agriculture and Forestry University, Xinyang 464000, China)
Abstract: In order to investigate the effects of adding resveratrol to the feed on the growth performance, health status and
ammonia nitrogen tolerance of Micropterus salmoides, the experiment was conducted on largemouth bass with an average body
mass of (7.40±0.05) g. The fish were randomly divided into three groups, each with three replicates of 30 fish each, and were
fed with experimental feeds containing 0.00%, 0.50% and 1.00% of resveratrol, and saturated feeding was carried out twice a
day for 56 d. The results showed that supplementing the diet with resveratrol significantly enhanced the weight gain rate, pro-
tein efficiency rate and food intake, and reduced the feed conversion ratio; increased the serum total protein (TP), albumin
(ALB) and globulin (GLB) contents, and decreased the triglyceride (TG), cholesterol (TC), alanine aminotransferase (ALT),
and aspartate aminotransferase (AST) contents; increased the activities of total antioxidant capacity (T-AOC), superoxide dis-
mutase (SOD), catalase (CAT), and glutathione peroxidase (GPX) in liver, decreased the contents of tumor necrosis factor-α
(TNF-α), interleukin-1 (IL-1), and interleukin-8 (IL-8); up-regulated the mRNA expression of superoxide dismutase (sod),
catalase (cat), glutathione reductase (gr), glutathione peroxidase (gpx), and b-cell lymphoma 2 (bcl-2) genes, and down-regu-
lated the expression of interleukin-1 (il-1), interleukin-8 (il-8), tumor protein 53 (p53), bcl-2 associated X protein (bax),
cysteine aspartate protease 3 (caspase3) and cysteine aspartate protease 9 (caspase9) genes. After 96 h of ammonia exposure,
the cumulative mortality and blood ammonia content in resveratrol group were significantly lower than those in control group;
but the serum lysozyme (LZM) activity, total complement (CH50), total immunoglobulin contents (IG), antibody titer (Ab
titer), the activities of argininosuccinate synthetase (ASS), argininosuccinate lyase (ASL), arginase (ARG), and ornithine car-
bamoyltransferase (OTC) in liver were significantly higher than those in control group. The study showed that the addition of
resveratrol to the feed could improve the growth performance, serum health, antioxidant capacity, inflammation and apoptosis
of largemouth bass, and that resveratrol could enhance the ammonia-nitrogen tolerance of largemouth bass by activating the
urea cycle, and improve the immune response to the occurrence of stress.
Key words: Micropterus salmoides; resveratrol; ammonia; stress resistance
Corresponding authors: FENG Dexiang. E-mail: dxfeng@xyafu.edu.cn;
LI Ming. E-mail: liming1@nbu.edu.cn

Funding projects: National Natural Science Foundation of China (32473130, 32072948); Zhejiang Provincial Department of
Agriculture and Rural Affairs Science and Technology Cooperation Project (2024SNJF059); Natural Science Foundation of
Ningbo (2024J172)

https://www.china-fishery.cn 中国水产学会主办 sponsored by China Society of Fisheries
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