Page 56 - 《水产学报》2025年第6期

金鲟，等 水产学报, 2025, 49(6): 069604

Genome-wide association analysis of low temperature tolerance in
Nibea albiflora

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JIN Xun , LIU Bingjian , ZHU Qihui , ZHANG Wanchang , LI Wanbo ,
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WANG Zhiyong , GAO Tianxiang , XU Dongdong 2*
1. National and Provincial joint Laboratory of Exploration and Utilizaion of Marine Aguatic Genetic Resources,
Marine Science and Technology College, Zhejiang Ocean University, Zhoushan 316021, China;
2. Key Lab of Mariculture and Enhancement of Zhejiang Provincial,
Zhejiang Marine Fisheries Research lnstitute, Zhoushan 316021, China;
3. School of Life Sciences, Nanchang University, Nanchang 330038, China;
4. Fisheries College, Jimei University, Xiamen 361000, China

Abstract: Increasing temperature extremes are caused by global climate change. Extreme low temperature has caused the
increase of death rate for Nibea albiflora in winter, which has seriously restricted the development of aquaculture industry. N.
albiflora is one of the important mariculture fish in China. Studying the molecular mechanism of response to low temperature
stress can provide theoretical basis for N. albiflora culture to cope with the challenges of extreme low temperature. In this
study, 200 N. albiflora individuals were selected to low-temperature stress at 5 °C, and the survival time, weight, body length,
sex and other phenotypic parameters of every individual were recorded. Then, whole genome was resequenced. A total of 3 005
715 high-quality single nucleotide polymorphism (SNPs) sites were obtained by comparing to the reference genome (NCBI
GenBank number: GCA_014281875.1), and the obtained SNP sites were used for genome-wide association analysis (GWAS)
using the Mixed linear model (MLM) based on EMMAX software. The results showed that the phenotypic parameters such as
body weight, body length and sex were not significantly correlated with the low temperature tolerance time (P=0.834-0.013).
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However, 9 sites were identified on 24 chromosomes that were significantly correlated (P=1.98×10 - 4.61×10 ) with the low
temperature tolerance time, and a total of 13 candidate genes, such as CBL.3, STOML2.2 and SLC37A3 were annotated. Gene
ontology (GO) analysis showed that the above genes were mainly enriched in the processes of immunity, defense and repair,
stress and mitochondrial energy metabolism, which may be related to low temperature response of the N. albiflora. The results
of this study can provide reference for the genetic improvement of low temperature tolerance.
Key words: Nibea albiflora; low-temperature stress; single nucleotide polymorphism (SNP); genome-wide association analysis
Corresponding author: XU Dongdong. E-mail: xudong0580@163.com
Funding projects: Zhejiang Province “Three Agriculture Nine Side” Science and Technology Cooperation Program Project
(2023SNJF078); Outstanding Youth Foundation of Zhejiang Province (LR21C190001); National Natural Science Foundation
of China (31972785)

https://www.china-fishery.cn 中国水产学会主办 sponsored by China Society of Fisheries
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