Page 159 - 《水产学报》2025年第6期

程庚，等 水产学报, 2025, 49(6): 069613

表 3 途径Ⅰ (2n♀+2n♂) 和途径Ⅱ (3n♀+2n♂) 诱导长牡蛎“海大 2 号”四倍体的效果 (诱导条件：低渗处理)
Tab. 3 Tetraploid induction efficiency between the pathway Ⅰ (2n♀+2n♂) and the pathway Ⅱ (3n♀+2n♂) of
C. gigas "Haida No.2" (induction condition: hypotonic treatment)
条件 卵裂率/% cleavage rate D幼率/% D larvae rate 四倍体率/% tetraploid rate
condition 2n♀+2n♂ 3n♀+2n♂ 2n♀+2n♂ 3n♀+2n♂ 2n♀+2n♂ 3n♀+2n♂
盐度 salinity
6 40.67±5.95 c 42.67±3.3 g 48.50±4.13 j 45.51±3.69 m 0 20.43±0.82 p
8 48.27±5.73 bc 44.49±0.83 g 55.39±4.86 ij 48.61±4.13 m 0 25.62±1.76 o
10 52.69±2.96 ab 50.51±2.18 f 57.47±5.13 i 52.42±6.47 lm 0 23.48±2.76 op
12 60.46±5.38 a 58.67±2.94 e 61.49±4.29 i 59.45±3.81 l 0 20.77±2.02 p
诱导持续时间/min
induction duration
10 56.66±3.94 d 52.76±4.20 h 47.61±6.04 k 45.31±4.83 n 0 20.66±2.92 r
15 53.16±1.26 d 52.67±1.16 h 45.53±1.67 k 46.35±1.28 n 0 23.72±1.76 qr
20 54.17±5.02 d 54.79±1.09 h 46.36±0.71 k 42.69±2.08 n 0 26.64±2.88 q
25 50.90±0.92 d 48.67±4.85 h 40.61±6.09 k 39.37±5.82 n 0 23.74±3.77 qr
对照组 control group 88.05±2.15 85.65±5.08 86.47±2.13 83.50±2.21 0 0

表 4 途径Ⅲ (2n♀+4n♂) 诱导长牡蛎“海大 2 号”四倍体的效果 (诱导条件：CB 和低渗处理)
Tab. 4 Tetraploid induction efficiency of the pathway Ⅲ (2n♀+4n♂) of C. gigas "Haida No.2"
(induction condition: CB and hypotonic treatment)
条件 卵裂率/% cleavage rate D幼率/% D larvae rate 四倍体率/% tetraploid rate
condition CB HT CB HT CB HT
CB/(mg/L)
0.25 71.60±3.88 a — 70.29±5.14 o — 23.32±2.16 b
0.50 62.30±5.92 b — 67.38±2.97 o — 45.57±3.37 A
0.75 50.35±4.03 c — 55.22±1.23 p — 56.36±4.18 z
1.00 42.30±1.13 d — 46.07±6.01 q — 55.61±5.22 z
盐度 salinity
6 — 41.39±3.99 k — 49.25±4.21 w — 20.42±0.97 ij
8 — 50.44±4.01 j — 60.21±7.95 v — 25.33±1.96 h
10 — 58.06±3.73 i — 65.31±5.28 v — 21.45±0.69 i
12 — 61.55±4.99 i — 68.48±0.82 v — 18.41±1.06 j
诱导时机/min
induction time
30% PB1 63.32±6.15 e 58.21±0.98 l 66.28±9.15 r 53.22±6.04 x 56.42±2.04 c 24.59±2.01 k
40% PB1 55.39±5.07 f 54.54±2.28 l 60.25±6.96 r 50.24±9.77 x 55.49±0.83 c 24.54±0.71 k
50% PB1 52.31±0.78 f 47.93±5.11 m 43.24±2.27 s 42.29±12.46 x 40.67±3.92 d 20.39±5.35 k
诱导持续时间/min
induction duration
10 67.42±3.79 g 52.68±1.05 n 63.44±2.89 t 51.43±1.86 y 32.41±3.93 g 0 m
15 65.50±4.32 g 54.23±8.54 n 64.29±4.21 t 51.53±3.27 y 44.34±2.11 f 20.67±5.84 l
20 60.48±6.08 gh 49.65±4.07 n 62.34±6.10 tu 50.37±6.15 y 60.45±3.19 e 25.51±3.93 l
25 56.27±2.21 h 45.47±4.30 n 53.2±8.24 u 44.68±10.81 y 50.71±7.09 f 21.63±2.94 l
对照组 control group 83.48±2.90 83.93±3.90 84.48±2.98 82.23±6.22 0 0
注：“HT”表示低渗处理；“—”表示无数据；下同。
Notes: "HT" means hypotonic treatment; "—" means that no data are available; the same below.

和 3N 对照组 (图 2-c)。 无法获得四倍体稚贝外，其他途径和处理方式
稚贝倍性组成 各处理组稚贝倍性组成 均可成功获得四倍体稚贝 (图 1-j)，并且与幼虫
情况显示，除途径Ⅰ(2n♀+2n♂) 下低渗处理组 阶 段 相 比 ， 四 倍 体 率 均 有 一 定 改 变 。 途 径

https://www.china-fishery.cn 中国水产学会主办 sponsored by China Society of Fisheries
6