Page 193 - 《水产学报》2023年第1期

孙东方，等 水产学报, 2023, 47(1): 019614

提示该位置可能是一些转录因子的结合位点。在 etogenic stages in triploid oysters Crassostrea gigas:
长牡蛎中，基因体中的 DNA 甲基化水平与基因表 irregular locking of gonial proliferation and subsequent
达成正相关，启动子区的 DNA 甲基化水平与基因 reproductive effort[J]. Journal of Experimental Marine
表达成负相关，但也存在正相关性 [15, 31] 。长牡蛎 Biology and Ecology, 2010, 395(1-2): 162-170.
Htatip2 的表达水平与 DNA 甲基化成负相关，这 [ 7 ] Piferrer F, Beaumont A, Falguière J C, et al. Polyploid
[38]
与癌症中的研究一致 ，同时也说明高保守性的 fish and shellfish: production, biology and applications
基因 DNA 甲基化的调控机制也比较保守。体外实 to aquaculture for performance improvement and genetic
验证明 Htatip2 启动子区发生甲基化抑制基因的表 containment[J]. Aquaculture, 2009, 293(3-4): 125-156.
[ 8 ] Spangenberg V, Arakelyan M, Galoyan E, et al. Reticu-
达。以上研究结果表明，三倍体不育型 (3nβ) 中
late evolution of the rock lizards: meiotic chromosome
DNA 甲基化可能抑制 Htatip2 的启动子活性，导
dynamics and spermatogenesis in diploid and triploid
致 Htatip2 表达水平降低，引起性腺发育过程中能
males of the genus Darevskia[J]. Genes, 2017, 8(6): 149.
量代谢障碍，最终导致卵子发生受阻。
[ 9 ] Dheilly N M, Jouaux A, Boudry P, et al. Transcriptomic
综上所述，本研究分析了长牡蛎 Htatip2 的
profiling of gametogenesis in triploid Pacific Oysters
组织表达以及不同性腺发育时期的基因表达模式，
Crassostrea gigas: towards an understanding of partial
分析了不同发育时期该基因的甲基化模式，并利
sterility associated with triploidy[J]. PLoS One, 2014,
用双荧光素酶报告验证了 DNA 甲基化对基因启动 9(11): e112094.
子活性的抑制作用。研究结果为深入细致地解析 [10] Yang Q, Yu H, Li Q. Disruption of cell division pre-
表观遗传对三倍体牡蛎不育机制奠定了基础。 vents gametogenesis in triploid Pacific oysters
(Crassostrea gigas)[J]. Aquaculture, 2022, 560: 738477.
（作者声明本文无实际或潜在的利益冲突） [11] Chen C, Yu H, Li Q. Investigation of the role of endo-
genous miRNAs in determining sterility in triploid
参考文献 (References)： Pacific oysters (Crassostrea gigas)[J]. Aquaculture,

[ 1 ] 农业农村部渔业渔政管理局, 全国水产技术推广总站, 2022, 561: 738606.
中国水产学会. 中国渔业统计年鉴–2022[M]. 北京: 中 [12] Sun D F, Yu H, Li Q. Genome-wide differential DNA
国农业出版社, 2022: 23-24. methylomes provide insights into the infertility of trip-
Fisheries and Fisheries Administration Bureau of the loid oysters[J]. Marine Biotechnology, 2022, 24(1): 18-
Ministry of Agriculture and Rural Areas, National 31.
Aquatic Technology Promotion Station, China Society of [13] Gavery M R, Roberts S B. Epigenetic considerations in
Fisheries. China fishery statistical yearbook-2022[M]. aquaculture[J]. PeerJ, 2017, 5: e4147.
Beijing: China Agriculture Press, 2022: 23-24 (in [14] Sun D F, Li Q, Yu H. DNA methylation differences
Chinese). between male and female gonads of the oyster reveal the
[ 2 ] Wadsworth P, Wilson A E, Walton W C. A meta-ana- role of epigenetics in sex determination[J]. Gene, 2022,
lysis of growth rate in diploid and triploid oysters[J]. 820: 146260.
Aquaculture, 2019, 499: 9-16. [15] Wang X T, Li Q Y, Lian J M, et al. Genome-wide and
[ 3 ] Dégremont L, Garcia C, Allen S K Jr. Genetic improve- single-base resolution DNA methylomes of the Pacific
ment for disease resistance in oysters: a review[J]. oyster Crassostrea gigas provide insight into the evolu-
Journal of Invertebrate Pathology, 2015, 131: 226-241. tion of invertebrate CpG methylation[J]. BMC Genom-
[ 4 ] Allen S K Jr, Downing S L. Performance of triploid ics, 2014, 15(1): 1119.
Pacific oysters, Crassostrea gigas (Thunberg). I. Sur- [16] Xiao H, Palhan V, Yang Y L, et al. TIP30 has an
vival, growth, glycogen content, and sexual maturation intrinsic kinase activity required for up-regulation of a
in yearlings[J]. Journal of Experimental Marine Biology subset of apoptotic genes[J]. The EMBO Journal, 2000,
and Ecology, 1986, 102(2-3): 197-208. 19(5): 956-963.
[ 5 ] Guo X, Allen S K Jr. Reproductive potential and genet- [17] Jiang C, Ito M, Piening V, et al. TIP30 interacts with an
ics of triploid pacific oysters, Crassostrea gigas (Thun- estrogen receptor α-interacting coactivator CIA and reg-
berg)[J]. The Biological Bulletin, 1994, 187(3): 309-318. ulates c-myc transcription[J]. Journal of Biological
[ 6 ] Jouaux A, Heude-Berthelin C, Sourdaine P, et al. Gam- Chemistry, 2004, 279(26): 27781-27789.
https://www.china-fishery.cn 中国水产学会主办 sponsored by China Society of Fisheries
8