Page 16 - 《渔业研究》2026年第1期

第 1 期 卢思宇等： 人工饲料驯化对翘嘴鳜肝脏代谢的影响 13

Effect of artificial diet domestication on the liver metabolism of
Siniperca chuatsi

3
1
2*
LU Siyu ，YU Hailuo ，LI Xujian ，LI Yaoguo 1*
(1. College of Fisheries, Hunan Agricultural University, Changsha 410128, China;
2. Wuhan Zhengda Fisheries Co. Ltd., Wuhan 430090, China;
3. Hubei Qianzhouhu Fisheries Co. Ltd., Xiantao 433099, China)

Abstract: [Background] As a famous and special aquatic breeding species, Siniperca chuatsi is prone to hep-
atobiliary diseases and other problems during the domestication process of artificial feed culture, which seri-
ously restricts the stable development of its industry. [Objective] This study aimed to explore the impact of
feed domestication on the growth and liver metabolism of S. chuatsi. [Methods] Taking S. chuatsi as the re-
search object, the entire process from fry stocking to successful domestication and artificial feed culture was di-
vided into three phases: the fry acclimation period, the artificial feed domestication period, and the artificial feed
culture period. A blood glucose meter was used to detect the blood glucose levels of the fish during the artificial
feed culture period, and growth-related traits of the fish were measured. Liver samples were randomly collected
from 8 S. chuatsi in the undomesticated period, the artificial feed middle culture period, and the late culture peri-
od, respectively for untargeted metabolomics detection and comparative analysis. [Results] The body mass and
body height of S. chuatsi measured on the 31st day of the artificial feed culture period were significantly higher
than those on the 22nd day (P<0.05). Compared with the undomesticated group, the artificial feed middle cul-
ture period group (16th day of the culture stage) had 915 differential metabolites, including 552 upregulated and
363 downregulated ones, the upregulated metabolites were mainly peptides and carboxylic acid derivatives, en-
riched in pathways such as metabolites pathways, amino acid biosynthesis, D-amino acid metabolism, and ABC
transporter. Compared with the undomesticated group, the late culture period group (31st day of the culture
stage) had 951 differential metabolites, with 551 upregulated and 400 downregulated ones, the upregulated
metabolites included peptides and carboxylic acid derivatives, enriched in pathways such as metabolic path-
ways, D-amino acid metabolism, ABC transporters, and protein digestion and absorption pathway. Compared
with the middle culture period group, the late culture period group had 228 differential metabolites, including
140 upregulated and 88 downregulated ones. Among them, glycylleucine and glycylphenylalanine and others
were significantly upregulated and enriched in glutathione metabolism; while prenol lipids long-chain fatty
acids and others were downregulated and enriched in pathways like metabolism pathways and ABC transporter.
[Conclusion] Artificial diet domestication of S. chuatsi is successfully carried out in a recirculating aquacul-
ture system. It is found that domestication increases the liver metabolic burden of S. chuatsi, possible reason lies
in changes in metabolic pathways involved in liver metabolism, such as glycolysis, the TCA cycle, D-amino
acid metabolism, and amino acid biosynthesis. Meanwhile, metabolic pathways including ABC transporters,
protein digestion and absorption, and glutathione metabolism play a role in alleviating the liver burden. This
study clarifies the effect of artificial feed domestication on the liver metabolism of S. chuatsi, providing support
for the domesticated culture of feed-fed S. chuatsi in recirculating aquaculture systems.
Key words: Siniperca chuatsi; artificial domestication; growth trait; liver health; metabolic change