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Chromosome preparation and karyotype analysis of gynogenetic diploids and
haploids in Ruditapes philippinarum

3
2
1,2
1,2*
1,2
LI Qiang , NIE Hongtao , WANG Bin , ZHOU He , HUO Zhongming , YAN Xiwu 1,2
1. Engineering Research Center of Shellfish Culture and Breeding in Liaoning Province,
Dalian Ocean University, Dalian 116023, China;
2. College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China;
3. Dalian Jintuo Aquatic Food Co., Ltd., Dalian 116400 , China

Abstract: Gynogenesis is an important breeding method for rapid establishment of pure lines. Karyotype analysis of gynogen-
etic offspring of Ruditapes philippinarum has not been reported. In order to clarify the karyotype of gynogenetic diploid, hap-
loid and normal diploid of R. philippinarum. In this experiment, the gynogenetic diploid, haploid and normal diploid larvae of
R. philippinarum were selected. The chromosome drops were made by air-drying technique, and the number of chromosomes
was counted and karyotype analysis was carried out. The results showed that the number of chromosomes in gynogenetic dip-
loid and normal diploid of R. philippinarum was 38, and the haploid was 19. The results of karyotype analysis showed that the
karyotype formula of gynogenetic diploid and normal diploid of R. philippinarum was 2n=18m +20sm, NF=76, and the hap-
loid karyotype was n=9m+10sm, NF=38. No sex chromosome and satellite were found in chromosome morphology. Studies
have shown that clams have only two types of chromosomes, m and sm. The karyotype of gynogenetic diploid is the same as
that of normal diploid, and the type of haploid chromosome is the same as that of normal diploid and the number is half of that
of diploid. In this study, the metaphase division phase and karyotype analysis results of gynogenetic diploid, haploid and nor-
mal diploid of R. philippinarum were obtained by air-drying technique using the trochophore larvae of R. philippinarum as
materials, which enriched the related research of gynogenetic and haploid chromosomes of R. philippinarum and the cognition
of chromosome number and karyotype composition of R. philippinarum. At the same time, it has important reference value for
the study of cytogenetics and genetic breeding of R. philippinarum.
Key words: Ruditapes philippinarum; gynogenesis; haploid; karyotype; trochophore; chromosome
Corresponding author: NIE Hongtao. E-mail: htnie@dlou.edu.cn
Funding projects: National Key Research and Development Program of China (2018YFD0901400); China Agricultural
Research System (CARS-49); the Liao Ning Revitalization Talents Program (XLYC2403165); the Research Project of Liaon-
ing Provincial Department of Education (LJ212410158027); the Outstanding Young Scientific and Technological Talents
Foundation of Dalian (2024RJ014)

中国水产学会主办 sponsored by China Society of Fisheries https://www.china-fishery.cn
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