Page 160 - 《水产学报》2025年第5期

边延欣，等 水产学报, 2025, 49(5): 059613

Effects of taurine on growth, glucolipids metabolism and
hepatic antioxidant of largemouth bass (Micropterus salmoides)

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BIAN Yanxin , XIE Kai , CAI Minglang , ZHU Bo , ZHOU Xixun ,
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HU Yi , ZHANG Junzhi 1*
1. Fisheries College, Hunan Agricultural University, Changsha 410128, China;
2. Yueyang Yumeikang Biotechnology Co., Ltd., Yueyang 414100, China
Abstract: To evaluate the effects of taurine on the growth performance, hepatic glucolipids metabolism, and hepatic antioxid-
ant capacities of largemouth bass (Micropterus salmoides), with an initial weight of (19.00±0.20) g were reared in cages (120
cm × 80 cm × 150 cm) for 8 weeks. The high starch control group (CON) was set at an 18% tapioca starch level, and 0.05% and
0.10% taurine (T0.05 and T0.1) were added based on this control group. The experiment consisted of three treatments, each
with three replicates of 50 fish per replicate. Results indicated that taurine addition to high-starch diets significantly increased
weight gain rate (WGR) and specific growth rate (SGR), while significantly decreasing liver crude fat and hepatic glycogen
content. Intestinal amylase activity was also significantly elevated. Histological staining with H.E, PAS, and oil red O revealed
that hepatocytes in the 0.1% taurine group were structurally intact, with reduced glycogen and fat droplets compared to the
CON group. Serum glucose, low-density lipoprotein cholesterol, glutamic oxalacetic transaminase (GOT), and glutamate pyr-
uvic transaminase (GPT) levels were significantly decreased after taurine addition, whereas alkaline phosphatase (ALP) and
high-density lipoprotein cholesterol (HDL-C) levels were significantly increased. Hepatic G6Pase activity was significantly
decreased in the taurine groups, while GK and PK activities were significantly elevated. The expression of genes related to gly-
colysis (pfk, gk and glut2) was up-regulated, while the expression of hepatic lipid synthesis-related genes (dgat1, lxr-1, srebp1,
acc1α, fasn and gpat4) was down-regulated. In contrast, the expression of lipolysis-related genes (hsl, mgl, pparα, lpl, atgl and
acadm) was up-regulated. Compared to the CON group, the expression of antioxidant-related genes (cat, sod, il-10 and tgf-β)
was up-regulated in the taurine gropus, while nf-κb and il-8 expression was down-regulated. In summary, adding taurine to high-
starch feeds can improve growth, regulate hepatic glucose-lipid metabolism, increase carbohydrate utilization, reduce hepatic
glycogen deposition and lipid accumulation, and enhance the antioxidant capacity of M. salmoides. Under the experimental
conditions, the recommended taurine addition in M. salmoides feed was 0.10%. This study provides a theoretical basis for taur-
ine's role in regulating glucose and lipid metabolism and offers technical support for improving carbohydrate utilization in
aquatic animals.
Key words: Micropterus salmoides; taurine; high-starch; glucolipid metabolism; antioxidant
Corresponding authors: HU Yi. E-mail: huyi740322@163.com;
ZHANG Junzhi. E-mail: zhjun123@hunau.edu.cn
Funding projects: National Nature Science Foundation of China (2019YFD0900200)

https://www.china-fishery.cn 中国水产学会主办 sponsored by China Society of Fisheries
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