Page 27 - 《水产学报》2023年第1期

陈松林，等 水产学报, 2023, 47(1): 019102

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Fish genome editing breeding in china: status, problems and prospects

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4
1,2*
1,2
CHEN Songlin , WANG Deshou , KUANG Youyi , CUI Zhongkai , LI Minghui 3
(1. Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Laboratory for Marine Fisheries Science and Food
Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China;
2. Key Lab of marine fisheries biotechnology and genetic breeding of Shandong Province, Qingdao 266071, China;
3. College of Life Science, Westsouthern University, Chongqing 400715, China;
4. Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, China)

Abstract: Genome editing breeding is a powerful technique in aquaculture organisms. China has made a big pro-
gress in genome editing researches and breeding in fish during last 10 years. The present paper is the first system-
atic review of the status, problems and prospect of fish genome editing breeding in China. Firstly, the authors gave
a brief introduction to advantages and disadvantages of three genome editing technologies including Zinc Finger
Nucleases(ZFN), Transcription Activator-Like Effector Nucleases(TALEN)and Clustered Regularly Interspaced
Short Palindromic Repeats CRISPR-Associated(CRISPR/Cas9). Secondly, the definition and workflow of genome
editing breeding in fish was proposed. Thirdly, the authors gave a detail introduction to the progress in genome
editing breeding in fish in China. In brief, Chinese scientists have carried out genome editing in several important
cultured fish such as tilapia, carp, crucian carp, half-smooth tongue sole, southern catfish et al. Especially, a vari-
ety of tilapia with sex-reversed female and with different color were generated by means of TALEN and
CRISPR/Cas9 method. Also, dmrt1-mutated F males with fast growth speed similar to females were generated by
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TALEN method in Chinese tongue sole. Crucian carp and blunt snout bream with less or no inter muscular bones
were obtained. These genome edited fish can mature and spawn. The progress has brought China's fish genome
editing breeding from following-up in the past to the present coexistence of following, running side-by-side, and
even leading in some fish species. Finally, the authors summarized the current shortcomings and problems in fish
genome editing breeding and prospected the future development direction of genome editing breeding in China,
and proposed research focuses in fish genome editing breeding. The paper will have important role and signific-
ance for fish genome editing breeding in China.
Key words: fish; genome editing breeding; status; problems; prospect

Corresponding author: CHEN Songlin. E-mail: chensl@ysfri.ac.cn
Funding projects: National Natural Science Foundation of China (31730099); CARS for Marine Fish Culture
Industry (CARS-47-G03); Central Public-interest Scientific Institution Basal Research Fund, CAFS (2020TD20);
Taishan Scholar Climbing Project Fund of Shandong, China

https://www.china-fishery.cn 中国水产学会主办 sponsored by China Society of Fisheries
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