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Study on function of scarb1 gene in Eriocheir sinensis and its

association with growth traits

JIANG Pengfei , HOU Xin , WANG Jun , CHEN Xiaowen , WANG Chenghui *
(Key Laboratory of Freshwater Aquatic Genetic Resources certificated by the Ministry of Agriculture and Rural Affairs/National
Demonstration Centre for Experimental Fisheries Science Education/Shanghai Engineering Research Center of Aquaculture,
Shanghai Ocean University, Shanghai 201306, China)

Abstract: In order to study the functions of class B type I scavenger receptor (SR-B1/SCARB1) in the metabolism,
growth, immunity and other biological processes of Eriocheir sinensis, the cloning and spatiotemporal expression
analysis of scarb1 gene in E. sinensis were analyzed, the changes of related tissue structure and carotenoid content
after RNA interference were observed, the SNP markers of the gene were screened and their association with the
growth related traits were evaluated. The results showed that the scarb1 gene in E. sinensis was composed of the
two exons and one intron. The open reading frame (ORF) was 2 415 bp and 805 amino acids were encoded; phylo-
genetic tree based on the amino acid sequences showed that the highest similarity with 80.51% were found
between E. sinensis and Portunus trituberculatus, indicating its high conservation among species. Its expression
was detected in all tissues encoded, including hepatopancreas, intestines, blood cells, heart, gills and muscle tis-
sues at different molting stages, but the relatively high expression existed in hepatopancreas, intestines and blood
cells. After RNA interference in scarb1 gene for 15 days and 30 days, histological observation showed that the
lumen of the hepatic tubules in hepatopancreas was blurred and partially vacuolized, and significant cavities
appeared in the muscular layer and submucosa of the intestinal intima. The color of hepatopancreas changed from
yellow to gray white, and the content of carotenoids decreased significantly. One SNP locus (C432T) was screened
in the first exon of the gene, which was significantly correlated with the weight gain rate and carapace length
growth rate after molting (P < 0.05). The results of this study provide useful guidance for genetic research of
scarb1 gene in E. sinensis and its breeding.
Key words: Eriocheir sinensis; Scarb1; gene expression; gene knockdown; SNP; growth traits
Corresponding author: WANG Chenghui. E-mail: wangch@shou.edu.cn
Funding projects: Agriculture Research System of Shanghai, China (Grant No2022-4)

https://www.china-fishery.cn 中国水产学会主办 sponsored by China Society of Fisheries
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