Page 149 - 《水产学报》2023年第1期

李晓晖，等 水产学报, 2023, 47(1): 019610

硫代谢 sulfur metabolism 硫代谢 sulfur metabolism
2 2
Ethe1 1 Ethe1 1
BPNT1 0 BPNT1 0
mTOR 信号通路 mTOR signaling pathway −1 mTOR 信号通路 mTOR signaling pathway −1
Seh1l −2 ddit4 −2
fzd3 log 2 倍数 Seh1l log 2 倍数
log 2 (Fold Change) log 2 (Fold Change)
ddit4 Lrp5
Lrp5 RPs6ka2
RPs6ka2 Slc38a9
SGK1 SGK1
Slc38a9 fzd3
萜类骨架的生物合成 terpenoid backbone biosynthesis 萜类骨架的生物合成 terpenoid backbone biosynthesis
icmt icmt
PCYOX1 PCYOX1
1-2
1-3
2-3
1-1
2-2
2-1
(a) 3-1 3-2 3-3 4-1 4-2 4-3 1-1 1-2 1-3 2-1 2-2 2-3 (b) 3-1 3-2 3-3 4-1 4-2 4-3
硫代谢 sulfur metabolism 硫代谢 sulfur metabolism
2 2
Ethe1 Ethe1
1 1
BPNT1 BPNT1
0 0
mTOR 信号通路 mTOR signaling pathway −1 mTOR 信号通路 mTOR signaling pathway −1
ddit4 −2 ddit4 −2
Lrp5 log 2 倍数 Lrp5 log 2 倍数
log 2 (Fold Change) log 2 (Fold Change)
fzd3 RPs6ka2
RPs6ka2 fzd3
Slc38a9 SGK1
SGK1 Seh1l
Seh1l Slc38a9
萜类骨架的生物合成 terpenoid backbone biosynthesis 萜类骨架的生物合成 terpenoid backbone biosynthesis
icmt icmt
PCYOX1 1-1 1-2 1-3 2-1 2-2 2-3 3-1 3-2 3-3 4-1 4-2 4-3 PCYOX1
4-2
4-3
4-1
2-1
2-2
1-1
1-2
1-3
3-2
3-3
2-3
3-1
(c) (d)
图 8 低氧胁迫下 3 个 KEGG 信号通路中 11 个鲢 miR-17a-5p 靶基因在肝脏 (a)、脑 (b)、
心脏 (c) 和鳃 (d) 中的相对表达量
1-1、1-2、1-3.对照组的 3 个重复，2-1、2-2、2-3.低氧组的 3 个重复，3-1、3-2、3-3.半窒息组的 3 个重复，4-1、4-2、4-3.窒息组的 3 个重复
Fig. 8 Relative expression of 11 miR-17a-5p target genes in 3 KEGG pathway liver (a),
brain (b), heart (c) and gill (d) under hypoxia stress
1. three replicates of normoxia group, 2. three replicates of hypoxia group, 3. three replicates of semi-asphyxia group, 4. three replicates of asphyxia group
miRNA 的表达与调控机制解析提供了参考，也为 Ruan W, Ji W W, Zheng L, et al. On hypoxia stress in
培育耐低氧的鲢新品系 (种) 提供了借鉴。 fish and its nutritional regulation and response[J]. Mar-
ine Fisheries, 2020, 42(6): 751 (in Chinese).
（作者声明本文无实际或潜在的利益冲突） [ 4 ] Huo D, Sun L, Ru X S, et al. Impact of hypoxia stress on
the physiological responses of sea cucumber
参考文献 (References)： Apostichopus japonicus: respiration, digestion, immunity
[ 1 ] Breitburg D. Effects of hypoxia, and the balance and oxidative damage[J]. Peerj, 2018, 6: e4651.
between hypoxia and enrichment, on coastal fishes and [ 5 ] Hou Z S, Wen H S, Li J F, et al. Environmental hypoxia
fisheries[J]. Estuaries, 2002, 25(4): 767-781. causes growth retardation, osteoclast differentiation and
[ 2 ] Feng C, Li X H, Sha H, et al. Comparative transcrip- calcium dyshomeostasis in juvenile rainbow trout (Onco-
tome analysis provides novel insights into the molecular rhynchus mykiss)[J]. Science of the Total Environment,
mechanism of the silver carp (Hypophthalmichthys 2020, 705: 135272.
molitrix) brain in response to hypoxia stress[J]. Compar- [ 6 ] Buentello J A, Gatlin D M, Neill W H. Effects of water
ative Biochemistry and Physiology D: Genomics & Pro- temperature and dissolved oxygen on daily feed con-
teomics, 2022, 41: 100951. sumption, feed utilization and growth of channel catfish
[ 3 ] 阮雯, 纪炜炜, 郑亮, 等. 鱼类低氧胁迫及营养调控和 (Ictalurus punctatus)[J]. Aquaculture, 2000, 182(3-4):
应对研究进展[J]. 海洋渔业, 2020, 42(6): 751. 339-52.
中国水产学会主办 sponsored by China Society of Fisheries https://www.china-fishery.cn
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