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Research progress on primordial germ cell development and reproductive
manipulation techniques of fish

1,2
TAO Binbin , HU Wei 1,2*
(1. State Key Laboratory of Freshwater Ecology and Biotechnology, Hubei Hongshan Laboratory,
Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China;
2. Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing 100101, China)

Abstract: Primordial germ cells (PGCs) are the earliest germline stem cells established during embryonic
development. The survival of species is dependent upon PGCs in sexually reproducing organisms. The spe-
cification of fish PGCs follows the pattern of “preformation” mode. In this mode, PGCs are specified by inher-
itance of maternal cytoplasm (germ plasm) during early embryogenesis. Specialized PGCs need to maintain
their cell fates and migrate over long distances to reach the gonadal primordium. The biological processes of
fish PGC specification, migration and fate maintenance are regulated by a variety of genes and signaling path-
ways. The study of fish PGC development is helpful for understanding the molecular mechanism of vertebrate
cell specification, migration, and fate maintenance, and it is also important for developing new reproductive
containment and germline stem cell transplantation techniques in farmed fish. Here, we review the research
progress in fish PGC development and reproductive manipulation techniques, and look into rospect of their
future development, hoping to provide theoretical and technical supports for the development of new tech-
niques applicable to creating new germplasm of important farmed fish.
Key words: fish; primordial germ cell; germ plasm; reproductive containment; germ stem cell transplantation
Corresponding author: HU Wei. E-mail: huwei@ihb.ac.cn
Funding projects: National Key R&D Program of China (2018YFD0901200); China Agriculture Research Sys-
tem of MOF and MARA (CARS-46); National Natural Science Foundation of China (31721005)

中国水产学会主办 sponsored by China Society of Fisheries https://www.china-fishery.cn
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