Page 120 - 《渔业研究》2026年第2期

第 2 期 苏 捷等： 腹泻性贝类毒素免疫荧光层析快速检测卡的测试与分析 263

Testing and analysis of rapid detection card for diarrheal shellfish poisoning by
immunofluorescence chromatography

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SU Jie ，WEN Jianxin ，HUANG Yiwen ，HU Rui 2
(1. Fisheries Research Institute of Fujian, Xiamen 361013, China;
2. Xiamen Higia Medical Technology Co., Ltd., Xiamen 361113, China;
3. Fujian Fishery Resources Monitoring Center, Fuzhou 350003, China)

Abstract: [Objective] Lipophilic toxins such as okadaic acid (OA) are the main components of diarrheal shell-
fish poisoning (DSP), which often cause food poisoning incidents and threaten the safety of the shellfish in-
dustry and consumer health. Traditional detection methods such as mouse bioassay and liquid chromatography-
tandem mass spectrometry (LC-MS/MS) are difficult to meet the needs of rapid screening at the grassroots
level. This study aims to test a newly developed rapid detection card based on immunofluorescence chromato-
graphy (IFC) technology, which can achieve rapid, sensitive, and efficient screening of OA and provide technic-
al support for monitoring departments. [Methods] By analyzing the test results of negative and positive
samples of Crassostrea gigas, Sinonovacula constricta, and Ruditapes philippinarum using the rapid detection
card, the sensitivity, accuracy, and precision of the rapid detection card were analyzed to evaluate its perform-
ance in detecting OA in shellfish.The consistency was verified through the comparison experiment with LC-
MS/MS. [Results] The limit of detection (LOD) and limit of quantification (LOQ) of OA in the three shellfish
species were 81.23 μg/kg and 191.07 μg/kg, respectively. The average recovery rate evaluated the detection ac-
curacy was (95.4±7.1)%, and the coefficient of variation used to evaluate the detection precision was between
0.03 and 0.11. The false positive and false negative rates were 0%. The linear regression analysis with LC-
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MS/MS showed a high correlation (R =0.957 8). [Conclusion] The IFC detection card has high sensitivity, spe-
cificity, stability, and is suer-friendly. It is suitable for rapid screening on-site and has important application
value in preventing and controlling shellfish toxin risks, ensuring shellfish food safety, and promoting the
healthy development of the shellfish industry.
Key words: diarrheal shellfish poisoning (DSP); okadaic acid (OA); immunofluorescence chromatography
(IFC); rapid detection