Page 128 - 《运动与健康科学》(英文)2024年第2期

TaggedAPTARAFigure
TaggedAPTARAEnd250 C.S. Norevik et al.
donor rat plasma (Fig. 3). Specifically, the cytokines granulo-
cyte macrophage colony stimulating factor (p = 0.018),
growth-regulated oncogene/keratinocyte chemoattractant
(GRO/KC) (p = 0.026), interleukin-1b (IL-1b)(p = 0.017),
IL-7 (p = 0.028), monocyte chemoattractant protein-1 (MCP-
1) (p = 0.004), macrophage inflammatory protein-1a (MIP-1a)
(p = 0.008), and macrophage inflammatory protein-3a (MIP-
3a)(p = 0.045) all had significantly lower concentrations in
ExPlas compared to SedPlas. Levels of granulocyte colony-
stimulating factor, interferon-g, IL-1a, IL-2, IL-4, IL-5, IL-6,
IL-10, IL-12 (p70), IL-13, IL-17A, IL-18, macrophage stimu-
lating factor, RANTES, tumor necrosis factor-a, and vascular
endothelial growth factor were not significantly altered by
Fig. 2. Cardiorespiratory fitness and weight in the wild-type donor rats. The exer- exercise training in the donor rat plasma (Supplementary
cised-trained donors ran on treadmills following a 10 £ 4 min HIIT protocol, Table 4).TaggedAPTARAEnd
5 times per week for 6 weeks, while the sedentary donors were not subjected to
exercise. After the 6-week period, all donors were weighed and cardiorespiratory
fitness was measured as VO 2peak (mL/kg 0.75 /min). (A) VO 2peak was significantly
higher in the exercise-trained donor rats (n = 15, all male) compared to the seden- TaggedAPTARAH23.5. Exercised plasma showed no effect on recognition
tary donor rats (n = 14, all male, *p = 0.028). (B) There was no significant differ- memory or contextual memory in AD ratsTaggedAPTARAEnd
ence in the weight of the exercise-trained and sedentary donor rats. All data are
presented as mean § SEM. HIIT = high-intensity interval training; SEM = standard TaggedAPTARAPA novel object recognition test and a 4-day fear condi-
tioning paradigm were used to evaluate the potential effects of
error of the mean; VO 2peak = peak oxygen uptake.TaggedAPTARAEnd
plasma injections on the cognitive function of AD rats
TaggedAPTARAH23.2. Six weeks of HIIT increased cardiorespiratory fitness in following the 6-week treatment period when started at either
an early, pre-plaque stage or at a later stage. There were no
donor ratsTaggedAPTARAEnd
significant differences between the early-stage treatment
TaggedAPTARAPFollowing the 6-week exercise paradigm, trained donor rats groups (Supplementary Fig. 6A) and later-stage treatment
had significantly higher VO 2peak values compared to sedentary groups (Supplementary Fig. 6B) in the preference for the
donor rats (Fig. 2A, p = 0.028). There was no significant differ- novel object over the familiar object and, hence, in recognition
ence in the weight of the 2 groups of donor rats measured right memory. Likewise, there were no significant differences in the
freezing behavior of the rats at any phase of the fear condi-
before the VO 2peak test (Fig. 2B).TaggedAPTARAEnd
tioning test (Supplementary Fig. 7).TaggedAPTARAEnd
TaggedAPTARAH23.3. HIIT had no effect on BDNF levels in plasma of donor ratsTaggedAPTARAEnd
TaggedAPTARAPThere were no significant differences in BDNF levels between
the ExPlas and SedPlas donor plasma (Supplementary Fig. 5).TaggedAPTARAEnd TaggedAPTARAH23.6. Plasma injections showed no harmful effects on the heartTaggedAPTARAEnd
TaggedAPTARAPEchocardiography measured from images taken in para-
TaggedAPTARAH23.4. Exercised donor plasma had reduced levels of several
sternal short axis view showed no volume overload or other
cytokinesTaggedAPTARAEnd
harmful effects on the heart following plasma injections.
TaggedAPTARAPThe multiplex immunoassay of 23 cytokines revealed that 7 There were also no differences between any of the treatment
cytokines were significantly altered by exercise training in the groups in terms of fitness (Supplementary Fig. 8).TaggedAPTARAEnd
TaggedAPTARAFigure

Fig. 3. Cytokines in exercised and sedentary donor plasma. Out of 23 cytokines, 7 were found at significantly lower concentration in ExPlas (n = 3, pooled plasma
from 16 donors) compared to SedPlas (n = 3, pooled plasma from 16 donors). The significantly altered cytokines included GM-CSF (p = 0.018), GRO/KC
(p = 0.026), IL-1b (p = 0.017), IL-7 (p = 0.028), MCP-1 (p = 0.004), MIP-1a (p = 0.008), and MIP-3a (p = 0.045). All data are presented as mean § SEM.
ExPlas = exercised plasma; GM-CSF = granulocyte macrophage colony stimulating factor; GRO/KC = growth-regulated oncogene/keratinocyte chemoattractant;
SedPlas = sedentary plasma; IL-1b = interleukin 1b; IL-7 = interleukin 7; MCP-1 = monocyte chemoattractant protein-1; MIP-1a = macrophage inflammatory
protein-1a; MIP-3a = macrophage inflammatory protein-3a; SEM = standard error of the mean.TaggedAPTARAEnd