Page 58 - 《水产学报》2026年第01期

1 期 缪煜琳，等：梨形环棱螺 Cyp17a1 基因的表达及功能 50 卷

1 taaaagccagtagttgataataatgtgtaggcctaatcagattgtgaatccagctgtgtgaatcagttcaacttagtcgaattattgcaa
91 aatagaaattagaattgagcagtaatgctctaacagagtatctgacagctccatcaaatttcgctggtttcggaagcagaatatcagtct
181 ttatcaacaaaatgcaagaggggtcgtcatttcttcgttgatatttctaagttggatgcacttagttatgttggcctgaaaagat ATGTC
1 M S
271 AGAGGTACTGACAGAAATGAAGGTGGCTCTCAGCCAAGGAATGGAATATATTAAAGAGTTGTCCGATCAGCTTCCTGGCACCGTAACACA
3 E V L T E M K V A L S Q G M E Y I K E L S D Q L P G T V T Q
361 GCAGGCCCTGATACTGGGGATGACTGTGGGTGTGGCAACTTATCTCCTATTGAAAAAGCATTATCACTTGCCACCAGGACCATTTAAACT
33 Q A L I L G M T V G V A T Y L L L K K H Y H L P P G P F K L
451 GCCTCTCGTGGGAAACCTGTTGTCTGTTATATACAGCAAAGAGCCATTTTACAAAACCCTATTAAGATGGTCAAGAGAGAAATATGGGCC
63 P L V G N L L S V I Y S K E P F Y K T L L R W S R E K Y G P
541 AGTCATCACAGTGTACATTGGTCCATTGAGATGTGTGACACTTAACCGCATTGATGTTGTCATGGAGGCATTAGTAAAAAAAAAGTCTGA
93 V I T V Y I G P L R C V T L N R I D V V M E A L V K K K S D
631 TTTTGCTGGACGACCATATTTATACTCTGTACACTCTTTTACAAATGGAGGAAAGGATATTGCCTTTACAACTTATTCATCCACTTGGAA
123 F A G R P Y L Y S V H S F T N G G K D I A F T T Y S S T W K
721 ACTTCACAAAACAATTGCAAGCCAGGCAGTCAGACACTTTATGACTGGACATCACTTTGAGCAGACAGTGCAAGATGTGGTTACCAAGAT
153 L H K T I A S Q A V R H F M T G H H F E Q T V Q D V V T K M
811 GATGAACAAGATGTCAGAAGAGAAGGACCCCTTTGATCCCAGTGGATATTTGACATCTCTCATGTTTACTATCCTTGATATATTTTGCTT
183 M N K M S E E K D P F D P S G Y L T S L M F T I L D I F C F
901 TGGTGTAAGCAAAGAACTGGATGATCCACAGCTCAAAAGAATCAGTTTTCTTTTTGATGAGTTCGGCAAGATAGTGGGCAATGGTTTTTG
213 G V S K E L D D P Q L K R I S F L F D E F G K I V G N G F W
991 GGAAGATCTGATTCCACCACTGAGGCTTTTTCCCACCAGAAAGTTTAAACTTTGTTCAGATAGTTTTCTTGAAATCAGAAATTATTTGCG
243 E D L I P P L R L F P T R K F K L C S D S F L E I R N Y L R
1 081 TCAAGAAATGAAGCGCCACAAGGACACATTCTCAGCTGACAACATCCGTGACATGGCAGACAGTATTTTGCTGGCTCAGCAAGAGATTGA
273 Q E M K R H K D T F S A D N I R D M A D S I L L A Q Q E I E
1 171 GAGGGAAGAGAACCCAGATGCCATGGCCATGATAACAGAAGACCATGTGGTAATGACAGTTGGGAATTTGTTTACAGCTGGTAGTGATAC
303 R E E N P D A M A M I T E D H V V M T V G N L F T A G S D T
1 261 AACCCGAGCAACGCTCTTGTGGGTGTTTTACTACCTTGCAGGACACCCAGAAGTGCAGAGTAAAGTTCAGGCTGAAATTGATGCTGCTAC
333 T R A T L L W V F Y Y L A G H P E V Q S K V Q A E I D A A T
1 351 AGAAGATCAGTTGCCTTCATTGTCAATGCGCTGCAAATTATCTTACACAGAAGCTATGTTGTATGAAGTCATGAGGATTGCGACGGTGGC
363 E D Q L P S L S M R C K L S Y T E A M L Y E V M R I A T V A
1 441 CCCTATTGGTGTTGCTCATACAACTATGTGTGATACCACCGTTGGTGGCTATGAAATCCCTAAGGGAACCATGGTGATCATCAACCACTG
393 P I G V A H T T M C D T T V G G Y E I P K G T M V I I N H W
1 531 GGCACTGCACAATGACCCAGACAAGTGGGACCAGCCTCATCTCTTCCGCCCAGAACGTTTTCTTGATAGTGATGGCAAACTGGTACCAAA
423 A L H N D P D K W D Q P H L F R P E R F L D S D G K L V P K
1 621 GCCTGAGAGCTGGCTTCCATTCTCTGCTGGTCATCGAGTGTGTCTTGGAGAGACAGTAGTTAAACCAGAGCTTGTACTTTTACTGACATT
453 P E S W L P F S A G H R V C L G E T V V K P E L V L L L T F
1 711 CATCTTGAAAAGACTTACTATTTCCTTGCCTCCAGGAGCTAAGTTTGACCCTCAGCCAATAGATAGCTTTTTAGCTGGGCCTCCAAAGCC
483 I L K R L T I S L P P G A K F D P Q P I D S F L A G P P K P
1 801 TTTCAAGATTGTTGTGACAGAACGGACTATAACATAAacaccacagtctgggtcttacctgtatggtgaaaatgtttgagaggtcataca
513 F K I V V T E R T I T *
1 891 cttcatttaatcacaatgacactttgaaatatacaaggctccgaatatcatggtggagatgataactacatggcaaatcttatgcgcaat
1 981 gtaaagcttttaagattacttataatactttacaaataaaatcactgtgcaacatttaaggtacaaacttctgatcaagatgaagggtgc
2 071 atatcatggtgcaagattaggaacaaacatgcaaatataagattaggaacatgaaaatcatggtgaaagattaggaacgaacatgcaaat
2 161 catggtgcaagattaggaatgaacatgcaaatcatggtgcaagat
图 1 Cyp17a1 基因核苷酸序列及氨基酸序列
小写字母表示部分 5' UTR 和 3' UTR；大写字母表示基因编码区 (上方为核苷酸序列，下方为氨基酸序列)；方框内为起始密码子和终止密
码子；下划线为亚铁血红素结合区。
Fig. 1 Nucleotide sequence and amino acid sequence of Cyp17a1
Lowercase letters indicate partial 5' UTR and 3' UTR; capital letters indicate the gene coding regions (nucleotides sequence above, amino acids sequence
below); the start codon and the stop codon are in the box; the iron heme binding region is underlined.

扰率达 49.72%；干扰 24 h 时，干扰率达 28.32%。 棱螺 12 h 后，检测梨形环棱螺性腺中睾酮和雌二
因此，合成的 2 条干扰链中，干扰链 2 组干扰梨 醇的含量。结果显示，干扰 Cyp17a1 基因后，雌
形环棱螺 12 h 时干扰效果最好 (图 5-b)。 雄螺体内睾酮含量均显著下降，雌二醇含量均极
选择干扰效果最好的干扰链 2 组干扰梨形环 显著上升 (图 6)。

中国水产学会主办 sponsored by China Society of Fisheries https://www.china-fishery.cn
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