Page 178 - 《水产学报》2025年第12期

徐成航，等 水产学报, 2025, 49(12): 129613

Effects of different selenium sources on growth performance,
serum biochemistry and intestinal antioxidant and inflammation in
bullfrogs (Rana catesbeiana)

2
1*
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XU Chenghang , ZUO Gang , CHEN Kaijian , HU Yi , ZHANG Junzhi 1*
1. College of Fisheries, Hunan Agricultural University, Changsha 410128, China;
2. Beijing Deyuanshun Biotechnology Co., Ltd., Beijing 102200, China
Abstract: In recent years, the bullfrog (Rana catesbeiana) farming industry has undergone rapid scaling and intensification. To
enhance production efficiency, feed formulations with a high soybean meal ratio have become a mainstream strategy. However,
anti-nutritional factors present in soybean meal can easily induce intestinal oxidative stress and inflammatory responses, com-
promising the immune defense system. This ultimately hinders the healthy and sustainable development of the bullfrog
industry. To investigate the effects of different selenium sources on growth performance, serum biochemistry, and intestinal
antioxidant and inflammation in under high soybean meal dietary conditions. A total of 840 healthy R. catesbeiana of the same
batch with an initial weight of (24.25±0.02) g were randomly divided into four groups with three replicates in each group. The
control (CON) feed, and the experimental feed with the addition of yeast selenium (Y0.8), sodium selenite (S0.8), and nano-sel-
enium (N0.8) at an effective level of 0.8 mg/kg to the control feed, were fed separately for 8 weeks. The results showed that all
three selenium sources promoted growth, improved survival rate, and reduced the feed coefficient. Among them, the Y0.8
group exhibited the highest weight gain rate, which was significantly increased by approximately 31.44% compared to the con-
trol group. The S0.8 group showed a significant increase of about 21.89%, while the N0.8 group showed an increase of about
10.88%, though not statistically significant. Regarding survival rate, both Y0.8 and S0.8 groups showed improvements without
significant differences, whereas the N0.8 group demonstrated a significant increase of about 15.24%. Additionally, the total
weight gain rate in the N0.8 group was significantly higher than that in the Y0.8 and S0.8 groups. Compared to the control
group, selenium supplementation effectively enhanced digestive enzyme activity and improved intestinal morphology. Mean-
while, GOT and GPT, as well as the levels TC and TG, were reduced in all experimental groups. Furthermore, intestinal antiox-
idant capacity was significantly enhanced in all selenium-supplemented groups, as evidenced by a significant decrease in
malondialdehyde (MDA) content, increased activities of antioxidant enzymes (GSH-Px, SOD, CAT, T-AOC), and upregula-
tion of related gene expressions (gpx5, sod, cat, gstp). Selenium supplementation also effectively suppressed the expression of
pro-inflammatory factors (il-8, il-1β) and promoted the expression of the anti-inflammatory factor il-10. The study indicates that
supplementation with all three selenium sources significantly improves growth performance and mitigates the adverse effects of
high soybean meal diets in R. catesbeiana. Nano-selenium demonstrated overall superior effects compared to selenium yeast
and sodium selenite. These findings provide theoretical and experimental support for the application of different selenium
sources in R. catesbeiana feed, offering positive implications for alleviating the negative impacts of high soybean meal diets,
improving R. catesbeiana health in aquaculture, and enhancing economic efficiency.
Key words: Rana catesbeiana; yeast selenium; sodium selenite; nano selenium; antioxidant capacity; alleviation of inflamma-
tion
Corresponding authors: HU Yi. E-mail: huyi740322@163.com;
ZHANG Junzhi. E-mail: zhjun123@hunau.edu.cn
Funding projects: Key Technology Development Services for the Bullfrog Full Industry Chain (XCZX-2023080); Hunan
Agricultural University Co-constructed National New Rural Development Research Institute (2022XCZX-395)

https://www.china-fishery.cn 中国水产学会主办 sponsored by China Society of Fisheries
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