Page 91 - 《水产学报》2025年第6期

李春，等 水产学报, 2025, 49(6): 069107

Effects of high temperature on the physiological metabolism and
antioxidant levels of Sinonovacula constricta

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LI Chun , LIU Qi , CHEN Yukuan , YUAN Li , NIU Donghong 1*
1. Shanghai Aquatic Animal Breeding and Green Breeding Cooperative Innovation Center,
Shanghai Ocean University, Shanghai 201306, China;
2. Sanmen County Aquatic Technology Promotion Station, Taizhou 317100, China

Abstract: Due to factors such as drought, tides, rainfall and human activities, the temperature of seawater in the nearshore
intertidal zone fluctuates greatly, and the organisms such as shellfish living there are susceptible. Therefore, in order to explore
the effect of high temperature stress on shellfish, the razor clam (Sinonovacula constricta) was used as the model organism in
this study. The control group (24 °C) and the high temperature groups (28 °C, 32 °C) with a stress time of 72 hours were set up
to start the comparative experiments. The survival rate, oxygen consumption rate and ammonia excretion rate of the S. con-
stricta were sampled at 0, 6, 12, 24, 48 and 72 h, respectively. The activity of superoxide dismutase (SOD) and the content of
malondialdehyde (MDA) in the hepatopancreas and gill of them were determined at the same time. The results showed that the
survival rate of S. constricta at 28 and 32 °C were 27.80%±4.15% and 20.00%±2.69%, respectively, which were significantly
lower than that at 24 °C 85.60%±1.60%. With the increase of temperature, the oxygen consumption rate and ammonia excre-
tion rate of S. constricta also increased, and reached the maximum value at 32 °C. SOD activity and MDA content in the hep-
atopancreas and gill tissues increased with the increase of temperature, and showed a trend of first increasing and then decreas-
ing with the extension of time. The results demonstrated that under acute high temperature stress, the physiological metabolic
activities of S. constricta intensified, but still maintained a certain survival rate and antioxidant capacity. The study can provide
a data reference for the cultivation of new strains of S. constricta with high temperature resistance.
Key words: Sinonovacula constricta; high temperature; survival rate; physiological metabolism; antioxidant level
Corresponding author: NIU Donghong. E-mail: dhniu@shou.edu.cn.
Funding projects: National Key Research and Development Program of China (2019YFD0900700); National Natural Science
Foundation of China (31472278); Provincial Policy Guidance Type Program (North Jiangsu Science and Technology Special
Project) (SZ-LYG202125)

中国水产学会主办 sponsored by China Society of Fisheries https://www.china-fishery.cn
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