Page 103 - 《水产学报》2025年第6期

张小明，等 水产学报, 2025, 49(6): 069108

Effects of hypoxic stress on energy metabolism, oxidative stress and
immune function in Onchidium reevesii

ZHANG Xiaoming 1,2,3 , TU Zhihan 1,2,3 , XIAO Haiming 1,2,3 , RAO Rongcheng 1,2,3 ,
CHEN Xilin 1,2,3 , QIAN Chang 1,2,3 , SHEN Heding 1,2,3*
1. National Demonstration Center for Experimental Fisheries Science Education,
Shanghai Ocean University, Shanghai 201306, China;
2. Shanghai Universities Key Laboratory of Marine Animal Taxonomy and Evolution,
Shanghai Ocean University, Shanghai 201306, China;
3. Shanghai Collaborative Innovation Center for Cultivating Elite Breeds and
Green-culture of Aquaculture animals, Shanghai Ocean University, Shanghai 201306, China

Abstract: This study explored the effects of hypoxic stress on energy metabolism, oxidative stress and immune function of
Onchidium reevesii. In this experiment, under hypoxic conditions [DO = (2.0 ± 0.2) mg/L], the changes of serum and hepato-
pancreatic biochemical indexes and the expression of hepatopancreas-related genes at 0, 4, 8, 12, and 24 h were measured. The
results showed that among the energy metabolism indexes, serum blood glucose, liver glycogen, serum and hepatopancreas
lactic acid levels all increased significantly with the passage of hypoxic stress. Among the antioxidant indicators, catalase
(CAT) activity and reduced glutathione (GSH) content showed an upward trend and then a downward trend. All reached the
maximum at 8 h, which were significantly higher than the control group. The content of malondialdehyde (MDA) showed an
upward trend with the passage of hypoxic stress and was significantly higher than that of the control group. Among the immune
indicators, alanine aminotransferase (ALT) and aspartate aminotransferase (AST) showed a fluctuating upward trend, and both
were significantly higher than the control group. And serum ALT and AST were higher than hepatopancreas from 8 h. The
expression of glycogen phosphorylase (GP) mRNA in hepatopancreas increased significantly under hypoxic stress. The expres-
sion of glutathione peroxidase (GSH-Px) mRNA and heat shock protein 70 (Hsp70) mRNA showed an upward trend and then a
downward trend, and all reached the maximum at 8 h, which was significantly different from the control group. Research
showed that O. reevesii mainly carries out anaerobic metabolism in order to adapt to the hypoxic environment, and hypoxic
stress affects the antioxidant capacity and immune function of the body.
Key words: Onchidium reevesii; hypoxic stress; energy metabolism; oxidative stress; immunity
Corresponding author: SHEN Heding. E-mail: hdshen@shou.edu.cn
Funding projects: Shanghai Collaborative Innovation Center Team Project (A1-3605-21-000202)

https://www.china-fishery.cn 中国水产学会主办 sponsored by China Society of Fisheries
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