Page 60 - 《水产学报》2025年第5期

陈涵，等 水产学报, 2025, 49(5): 059605

Effects of acute alkalinity stress on anti-stress, antioxidant capacity and
muscle tissue structure of Eriocheir sinensis

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CHEN Han , QIU Qiyong , WANG Meiyao 1,2*
1. Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China;
2. Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs,
Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China

Abstract: China possesses abundant saline-alkali water resources that are currently underutilized. Developing saline-alkali
water aquaculture could transform these abandoned resources into valuable assets and enhance fishery production. This study
aimed to investigate the effects of acute alkalinity stress on the physiological metabolism of Eriocheir sinensis. A control group
(0 mmol/L) and an alkalinity group (60 mmol/L) were established, with four treatment times (0, 6, 12, and 24 h) to examine the
impacts of acute alkalinity stress on the mRNA expression of anti-stress and developmental regulatory genes (mTOR and AKT)
in the hepatopancreas of E. sinensis, as well as antioxidant capacity indicators [catalase (CAT), total antioxidant capacity (T-
AOC), malondialdehyde (MDA)] and muscle tissue structure, which are key tissues for energy metabolism. Results showed that
after 6 h of alkalinity stress, AKT gene expression increased and then decreased, but remained significantly higher than the con-
trol group at 24 h (P<0.05). mTOR expression significantly increased after 6 h of stress (P<0.05) and remaind highly
expressed. CAT enzyme activity in the hepatopancreas of E. sinensis significantly decreased after 6 h of stress (P < 0.05). T-
AOC levels in the hepatopancreas significantly increased after 24 h of alkalinity stress (P<0.05), while MDA content signific-
antly increased with prolonged stress, peaking at 24 h (P<0.05). Muscle tissue structure in E. sinensis was not significantly
affected by 6 h of alkalinity stress. The observed increases in antioxidant enzyme activity (T-AOC) and upregulation of stress-
resistant and developmental genes (AKT and mTOR) indicated that E. sinensis actively responded to alkalinity stress. This
study provides theoretical guidance for eluciating the anti-stress mechanisms of E. sinensis in saline-alkali environments and for
the future development of E. sinensis aquaculture.
Key words: Eriocheir sinensis; alkalinity stress; hepatopancreas; antioxidant capacity; anti-stress
Corresponding author: WANG Meiyao. E-mail: wangmeiyao@ffrc.cn
Funding projects: Jiangsu Revitalization of Seed Industry (JBGS〔2021〕031); Central Public-Interest Scientific Institution
Basal Research Fund, CAFS (2023TD39); National Key R & D Program of China (2022YFD2400702); Central Public-interest
Scientific Institution Basal Research Fund, Freshwater Fisheries Research Center, CAFS (2023JBFM05)

https://www.china-fishery.cn 中国水产学会主办 sponsored by China Society of Fisheries
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