Page 207 - 《水产学报》2023年第1期

操文杰，等 水产学报, 2023, 47(1): 019615

Research on the allogynogenetic biological effects in the second generation
gynogenetic of Carassius auratus var. pengsenensis induced with
sperms from Elopichthys bambusa and Culter alburnus

CAO Wenjie , ZHANG Jingrong , ZHANG Qingfei , ZHAO Yuhua , WANG Weimin *
(Key Lab of Freshwater Animal Breeding, Ministry of Agriculture and Rural Affairs, College of Fisheries,
Huazhong Agricultural University, Wuhan 430070, China)

Abstract: The technology of allogynogenesis has great application potential in the breeding of superior varieties.
Carassius auratus var. pengsenensis is a kind of hermaphroditic triploid crucian carp with natural gynogenetic
reproduction and it is one of the best freshwater breeding varieties in China. The development of gynogenesis of C.
auratus var. pengsenensis induced with heterologous sperms can produce normal surviving progeny. The study on
allogynogenetic biological effects in progeny can provide reference for the cultivation of excellent new varieties of
C. auratus var. pengsenensis. In this study, the eggs of C. auratus var. pengsenensis were fertilized with sperms
from Elopichthys bambusa and Culter alburnus for two successive generations, respectively. We succeeded in
obtaining their second generation offsprings, P×G (C. auratus var. pengsenensis ♀ × E. bambusa ♂) and P×Q (C.
auratus var. pengsenensis ♀ × C. alburnus ♂). The chromosomal karyotype, DNA content, morphological traits,
and growth performance of offsprings were compared with P×P (C. auratus var. pengsenensis). The findings sug-
gest that the karyotype formulas of P×G, P×Q and P×P was 3n=150=45m+66sm+27st+12t,
3n=150=54m+51sm+39st+6t and 3n=150=51m+48 sm+45st+6t, respectively. It is significantly different from the
karyotype of E. bambusa (2n=48=10m+24sm+12st+2t) and of C. alburnus (2n=48=16m+26sm+6st). Furthermore,
observed ratio of nuclear DNA content of P×G and P×Q to nuclear DNA content of P×P was within the 95% con-
fidence interval (both 0.97), showing that P×G and P×Q produced by heterologous sperms were gynogenetic trip-
loid fishes. The findings of principal component analysis based on morphological parameters reveal almost no
overlap between the scatter plots of P×Q and P×G and P×P; there seem to be significant morphological differences
between P×Q and P×G and P×P (P<0.05) that can be made preliminary discrimination by discriminant function
(the accuracy rate reached 97.8%). These findings suggest that there is a significant allogynogenetic biological
effects between P×Q and P×G progeny. As compared to P×P, P×G and P×Q demonstrated benefits in terms of
mean body weight, mean body length, body weight growth rate and body length growth rate at each age, and P×G
had significant benefits (P<0.05). In conclusion, sperms from both E. bambusa and C. alburnus can promote gyno-
genic development of C. auratus var. pengsenensis eggs, and different sperm sources have varied effects on the
development of gynogenic nucleus and progeny, as expected from allogynogenetic biological effects. Through the
research results, we can preliminarily judge the better sperm stimulation source, which lays the foundation for the
subsequent breeding of C. auratus var. pengsenensis excellent new varieties.
Key words: C. auratus var. pengsenensis; gynogenesis; allogynogenetic biological effects; karyotype; morpho-
logy; growth performance
Corresponding author: WANG Weimin. E-mail: wangwm@mail.hzau.edu.cn

Funding projects: Modern Agricultural Industrial Technology System Construction of Jiangxi Province (JXARS-
03)

https://www.china-fishery.cn 中国水产学会主办 sponsored by China Society of Fisheries
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