Page 114 - 《水产学报》2025年第8期

林悦彤，等 水产学报, 2025, 49(8): 089310

plaque adhesion strength, as well as the secretion Taishan Island (26°58 ′43 ″N, 120°43 ′03 ″E) in
number of byssal threads. The results of this study Fuding (FD). Upon laboratory arrival, the mus-
provide valuable information into genetic breeding sels were cleaned of surface attachments and
aimed at enhancing the byssus attachment in M. acclimated in 10 L polycarbonate tanks contain-
ing seawater (30 PSU) for a week at 21℃. A mix-
coruscus. This is the first study to demonstrate dif-
ture of microalgae, composed of Platymonas hel-
ferences in byssal thread phenotypic characteristics
golandica var. tsingtaoensis and Isochrysis zhanji-
and genetic variability among three geographic pop-
angensis were fed daily. The seawater in the tanks
ulations of M. coruscus. This information will be
was changed every other day. Mussel gill samples
helpful for the selection of parental mussels in the
were collected for DNA extraction and stored at
breeding practice of M. coruscus.
−40℃. The genomic DNA of gill tissue was extrac-

ted using the TIANamp Marine Animals DNA Kit
1 MATERIAL AND METHODS
(Tiangen Biotech Co., Ltd., Beijing, China) and

diluted to 10 ng/μL for polymerase chain reaction
1.1 Sample collection and DNA extraction
(PCR).

All animal experiments were conducted in
1.2 Microsatellite analysis
accordance with the guidelines and approval of the
respective Animal Research and Ethics Committees Ten high polymorphism microsatellite loci
of Shanghai Ocean University (Approval No. (MC08, MC18, MC44, MC47, MC54, MC57,
SHOU-DW-2021-011). The operators strictly fol- MC66, MC74, MC76, and MC99) and their pairs of
[18]
lowed the ethical guidelines during the experiment. primers selected from previous research were
used in the present study. The genotyping of each
A total of 158 wild mussels M. coruscus were col-
locus for all the samples was electrophoresed on an
lected from 2022 to 2023 in three geographic areas
ABI 3730XL DNA sequencer and alleles sizes were
covering the distribution of the natural populations
analyzed by using GeneMapper Version 3.5 soft-
in China (Fig. 1). Of these, two wild populations
ware (Applied Biosystems).
from Zhejiang Province were collected from
The number of alleles (N ), Shannon Wiener
a
Langgang Island (30°26 ′16 ″N, 122°56 ′15 ″E) in
index (I), observed heterozygosity (H ), expected
o
Shengsi (SS) and Yangqi Island (28°24 ′49 ″N,
heterozygosity (H ), pairwise population differenti-
e
121°54′50″E) in Jiaojiang (JJ). Another wild pop-
ation (F ) and private alleles (P ) for each
ST
a
ulation from Fujian Province was collected from
microsatellite locus were calculated using
N GenALEx Version 6.5 . Deviations from Hardy-

[19]
Weinberg equilibrium (HWE) at each locus for each
Shengsi
30° (30°26′16″N, 122°56′15″E) population were tested using GENEPOP Version
[20]
4.7 . The alleles richness (A ), and inbreeding
r
coefficient (F ) at each locus for the three popula-
is
[21]
tions were evaluated using FSTAT Version 2.9.3 .
The polymorphic information content (PIC) was
Jiaojiang [22]
28° (28°24′49″N, 121°54′50″E) estimated by Cervus Version 3.0 . Analysis of
molecular variance (AMOVA) based on allele fre-
0 50 km quency information was calculated using Arlequin
Fuding
[23]
(26°58′43″N, 120°43′03″E) Version 3.5 .
120° 122° E GenALEx Version 6.5 was used to express
Fig. 1 Map of three sampling sites of three wild genetic distance between three populations based on
[24]
populations of M. coruscus the mean of Nei’s unbiased genetic distance , and
中国水产学会主办 sponsored by China Society of Fisheries https://www.china-fishery.cn
3