Page 186 - 《水产学报》2025年第6期

梅静，等 水产学报, 2025, 49(6): 069615

Adaptive responses of Hyriopsis cumingii to different purple soils under
high-temperature stress

MEI Jing , YANG Qinglin , TANG Xiaoqi , LIU Yang ,
LI Yanhong , YU Xiaobo , WU Zhengli *
Key Laboratory of Freshwater Fish Reproduction and Development, Ministry of Education,
Research Center of Fishery Resources and Environment, College of Fisheries,
Southwest University, Chongqing 400715, China

Abstract: Soil sediment constitutes a vital element within pond aquaculture systems, exerting a profound influence on the
growth and disease resistance of cultivated animals. In recent years, aquaculture has been under severe threats due to extreme
thermal weather conditions. To scrutinize the impact of different sediment types on the high-temperature stress resistance of
Hyriopsis cumingii, this study constructed a 28-day aquaculture experiment employing three different purple soils (S1, Feixian-
guan group; S2, Shaximiao group; S3, Suining group) as sediment. Regular assessments of water quality indicators [ammonia
+
−
−
nitrogen (NH 4 -N), nitrite nitrogen (NO 2 -N), nitrate nitrogen (NO 3 -N), total nitrogen (TN), total phosphorus (TP), active
phosphorus (AP), chemical oxygen demand (COD), total hardness (TH)] across varied aquaculture systems were conducted.
After the completion of the aquaculture phase, H. cumingii in diverse systems were subjected to ambient (23 °C) and high-tem-
perature (33 °C) conditions. Hemolymph antioxidant enzymes [superoxide dismutase (SOD), catalase (CAT)], immune-related
enzymes [alkaline phosphatase (AKP), lysozyme (LZM)] activities and H 2 O 2 levels were measured at 6 h and 12 h after expos-
ure, and the expression of hepatopancreas immune related genes (IAP, IL-17, HSP70) was analyzed. The results revealed differ-
ences in water quality indicators among the three aquaculture systems during the cultivation of H. cumingii. The system using
S3 purple soil as sediment had the highest TP levels (peak value: 1.13 mg/L) and lower NO 2 -N concentrations (peak value: 0.12
mg/L) compared to the other systems, resulting in a more favorable environment for the growth and health of the cultured
organisms. Following high-temperature stress, the activities of SOD and CAT in H. cumingii cultured in S3 purple soil
increased to 1.2- to 2.0-fold of those in the control group, and the expression of IAP, IL-17, and HSP70 genes was significantly
up-regulated (1.2- to 13.0-fold), indicating enhanced thermotolerance. The adaptability hierarchy among distinct bottom sedi-
ment aquaculture systems, in terms of resisting high-temperature stress in H. cumingii, was ranked as S3 > S1 > S2. These find-
ings contribute to a more profound comprehension of the influence of aquaculture sediment on the high-temperature resistance
of aquatic organisms.

Key words: Hyriopsis cumingii; purple soil; physical and chemical properties; high-temperature stress
Corresponding author: WU Zhengli. E-mail: zh20140202@swu.edu.cn
Funding projects: Graduate Scientific Research and Innovation Foundation of Chongqing (CYS223236); Chongqing Natural
Science Foundation (CSTB2022NSCQ-MSX0305); Chongqing Ecological Fisheries Industry Technology System
(CQMAITS202315)

中国水产学会主办 sponsored by China Society of Fisheries https://www.china-fishery.cn
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