Page 26 - 《渔业研究》2026年第1期

第 1 期 李 娴等： 乌鳢生长性状全基因组关联分析 23

Genome-wide association study of growth traits in Channa argus

LI Xian ，WANG Ya ，CAO Xiao ，ZHU Shuren ，AN Li ，ZHU Yong’an ，
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MENG Qinglei ，DONG Wen ，DONG Jun 1
(1. Shandong Freshwater Fisheries Research Institute, Jinan 250013, China;
2. Fisheries Development Service Center of Weishan County, Jining 277699, China)
Abstract: [Objective] Channa argus is an important freshwater economic fish in China. In recent years, due to
environmental impacts and breeding methods, the genetic resources of C. argus have gradually deteriorated.
This study aims to investigate the genetic basis of growth traits in C. argus using genome-wide association stud-
ies (GWAS) to provide a basis for the conservation of genetic resources and the selection of superior breeds in
C. argus. [Methods] A total of 405 individuals of C. argus were subjected to reduced-representation genome
sequencing using the Illumina Nova (PE150) platform and Super-GBS technology. The GWAS analysis was
performed using the efficient mixed model of the EMMAX software to identify SNPs significantly associated
with growth traits. Sequences within 50 kb upstream and downstream of the significant loci were scanned to
identify potential candidate genes. The biological functions of the candidate genes were annotated based on the
NCBI database and literature search results, and candidate functional genes related to the target traits were
screened. [Results] After filtering the sequencing data, a total of 47 536 SNP loci were obtained. GWAS ana-
lysis identified three SNPs significantly associated with body mass traits: 2831060, 2830864, and 2830886. By
scanning sequences within 50 kb upstream and downstream of the significant loci, seven candidate functional genes
related to body mass in C. argus were annotated. In addition, six potential SNPs associated with total length
traits were identified, and 17 potential candidate functional genes related to total length traits were annotated.
Among these functional genes, seven were associated with both total length and body mass traits, namely irak3,
tuba1a, hspa14, prl, rint1, helb and net1. These functional genes are mainly involved in biological processes
such as growth metabolism, developmental regulation, cell proliferation, and immune regulation in C. argus.
[Conclusion] The identified SNPs and functional genes are significantly associated with body mass and total
length traits in C. argus, providing important references for the mechanism analysis of growth traits, superior
breed selection, and resource conservation in C. argus.
Key words: Channa argus; genome-wide association study (GWAS); growth traits; Super-GBS