Page 66 - 《水产学报》2026年第01期

1 期 缪煜琳，等：梨形环棱螺 Cyp17a1 基因的表达及功能 50 卷

Expression and functional study of Cyp17a1 in Bellamya purificata

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MIAO Yulin , LÜ Huohong , GU Yang , JIN Xin , WANG Ping ,
WANG Guiling 1,2,3* , LI Jiale 1,2,3
(1. Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs,
Shanghai Ocean University, Shanghai 201306, China;
2. Shanghai Engineering Research Center of Aquaculture, Shanghai Ocean University, Shanghai 201306, China;
3. Shanghai Collaborative Innovation for Aquatic Animal Genetics and Breeding,
Shanghai Ocean University, Shanghai 201306, China)

Abstract: Bellamya purificata, a unique edible freshwater snail in China, has considerable economic value. To study the role of
the steroidogenic enzyme gene Cyp17a1 in the hormone synthesis process of B. purificata, this study cloned the open reading
frame (ORF) region of the Cyp17a1 gene. The expression patterns of Cyp17a1 in B.purificata were analyzed using quantitative
real-time PCR and in situ hybridization. RNA interference was performed on the Cyp17a1 gene, and B. purificata was
immersed in different concentrations of hormones to explore the role of Cyp17a1 in the synthesis of steroid hormones. The res-
ults showed that the ORF region of Cyp17a1 in B. purificata is 1 572 bp in length, encoding 523 amino acids. The expression of
Cyp17a1 varied across different tissues of B. purificata, with significantly higher expression in males than in females, except in
the gills. The gene was primarily expressed in spermatocytes and spermatids in the testes. After 12 hours of RNA interference
with Cyp17a1 in the gonads, testosterone levels significantly decreased in both males and females, while estradiol levels signi-
ficantly increased. Immersion in different concentrations of hormones for 24 days revealed that treatment with 40 ng/L of 17β-
estradiol (E2) and 17α-methyltestosterone (MT) resulted in an initial decrease followed by an increase in Cyp17a1 expression.
In contrast, treatment with 200 ng/L of E2 and MT led to an initial increase followed by a decrease in Cyp17a1 expression.
These findings indicate that Cyp17a1 is involved in the synthesis and transformation of steroid hormones in B. purificata. This
study provides valuable insights into the steroid hormone synthesis process in B. purificata and lays a theoretical foundation for
research on sex regulation and gonadal development in this species.
Key words: Bellamya purificata; Cyp17a1; 17 beta-estradiol; 17 alpha-methyltestosterone; RNA interference (RNAi)
Corresponding author: WANG Guiling. E-mail: glwang@shou.edu.cn
Funding projects: National Key Research and Development Program of China (2022YFD2400700); National Natural Science
Foundation of China (32573497)

中国水产学会主办 sponsored by China Society of Fisheries https://www.china-fishery.cn
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