Page 81 - 《水产学报》2026年第01期

1 期 李文升，等：豹纹鳃棘鲈工厂化室内繁育及早期生长发育特征 50 卷

Industrial indoor breeding, early growth and development characteristics of
Plectropomus leopardus

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LI Wensheng , GUO Xiaoli , YANG Chuanjun , HE Xinlei , PANG Zunfang ,
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MA Wenhui , ZHANG Yingguang , SUN Fangfang , ZHANG Jiawei ,
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YUAN Songzhu , ZHANG Quanqi , CHEN Songlin 2*
(1. Fisheries College, Ocean University of China, Qingdao 266071, China;
2. State Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute,
Chinese Academy of Fishery Sciences, Qingdao 266071, China;
3. College of Life Sciences, Qingdao University, Qingdao 266071, China;
4. Laizhou Mingbo Aquatic Co., Ltd., Yantai 261418, China)
Abstract: This study accomplished the fully industrialized indoor breeding of Plectropomus leopardus and investigated the
characteristics and patterns of embryo and early post embryonic development of P. leopardus in the north indoor factory work-
shops. This study used microscopic imaging and morphological measurement observation methods to track the early develop-
mental processes and characteristics of fertilized eggs of P. leopardus obtained through indoor factory cultivation and natural
reproduction. We found the zygotes were transparent floating spherical eggs with an average diameter of (887.08±10.28) μm
and a single oil bulb with an average diameter of (166.60±10.81) μm. Embryonic development experience 8 classical develop-
mental stages, including fertilized egg stage, blastodisc stage, cleavage stage, blastula stage, gastrula stage, neurula stage,
organo-forming stage, and hatching stage, subsequently divided into 27 stages. The fertilized eggs were hatched at 27 h 13 min
after fertilization followed by the larval stage. The total length of the newly hatched larvae was (2.156±0.281) mm. According
to the absorption of yolk sac, larval stage was further divided into pre-larval stage [0-3 (days post hatching, dph), not complete
absorption of yolk sac] and post-larval stage (4-30 dph, complete absorption of yolk sac). The oil globule disappeared at 4 dph
and the swimming bladder appeared at 8 dph. At 28 dph, the total length (15.32±1.98) mm, the length of the second dorsal fin
spine (8.04±1.06) mm, and the length of the first pelvic fin (5.97±1.37) mm, extended to the maximum value. Afterwards, the
length began to shorten. When the dorsal fin spine contracted to the minimum value (3.26±1.13) mm, all kinds of fins were
fully formed, scales began to appear and body color began to deepen, marking the entry into the juvenile stage. At 71 dph,
entering the adult fish, the total length (40.21±1.33) mm，the body shape was similar to that of the adult fish and the whole
body was covered with scales and baby blue spots. For the first time, this study achieved the natural reproduction spawn and
artificial cultivation of P. leopardus in the northern industrial workshop, revealing its early growth and development character-
istics and pattern,laying a theoretical and technical basis for the the full factory batch cultivation of leopard coral grouper seed-
lings.
Key words: Plectropomus leopardus; fertilized egg; embryonic development; post-embryonic development
Corresponding authors: ZHANG Quanqi. E-mail: qzhang@ouc.edu.cn;
CHEN Songlin. E-mail: chensl@ysfri.ac.cn
Funding projects: National Major Project for Biological Breeding (2023ZD0405502); Key Research and Development Project
of Shandong Province (2023ZLYS02); Innovation Team Project of Chinese Academy of Fishery Sciences (2023-TD-20); Shan-
dong Taishan Scholar Climbing Project; Hainan Province Science and Technology Special Fund (ZDYF2024XDNY278); Aca-
demician Chen Songlin’s Workstation of Hainan Province (YSGZZ2023001); Innovation Platform for Academicians of Hainan
Province (YSPTZX202402)

中国水产学会主办 sponsored by China Society of Fisheries https://www.china-fishery.cn
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